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Regenerating axolotl retinas regrow diverse cell types with modulation by Notch signaling and reconnect to the brain

ABSTRACT: Regenerating axolotl retinas regrow diverse cell types with modulation by Notch signaling and reconnect to the brain

PROVIDER: PRJNA824487 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18675650_1.fastq.gz	Fastqsanger.gz
	SRR18675650_2.fastq.gz	Fastqsanger.gz
	SRR18675651_1.fastq.gz	Fastqsanger.gz
	SRR18675651_2.fastq.gz	Fastqsanger.gz
	SRR18675652_1.fastq.gz	Fastqsanger.gz

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Regenerating axolotl retinas regrow diverse cell types with modulation by Notch signaling and reconnect to the brain

Project description:We examined uninjured and regenerating axolotl salamander retinas, including their transcriptional profile. We report that at 27 days after a retinectomy (early-mid regeneration), regenerating axolotl retinas exhibit downregulation of genes associated with neuronal networks but upregulation of genes associated with angiogenesis and the extracellular matrix. We also report differential expression of two Notch pathway effector genes, Hes1 and Hes5.

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Single-cell level transcriptomic anaylsis of the homeostatic and regenerating axolotl limb

Project description:Regeneration of complex multi-tissue structures, such as limbs, requires the coordinated effort of multiple cell types. In axolotl limb regeneration, the wound epidermis and blastema have been extensively studied via histology, grafting, and bulk-tissue RNA-sequencing. However, studying the contributions of these tissues is hindered due to limited information regarding the molecular identity of the cell types in regenerating limbs. By performing unbiased single-cell RNA-sequencing on over 25,000 cells from axolotl limbs, we identify a plethora of cellular diversity within epidermal, mesenchymal, and hematopoietic lineages in homeostatic and regenerating limbs. We identify regeneration-induced genes, develop putative trajectories for blastema cell differentiation, and propose the molecular identity and origin of fibroblast-derived blastema progenitor cells residing in homeostatic limbs. This work will enable application of molecular techniques to assess the contribution of these populations to limb regeneration. It will also facilitate work aimed at identifying transcripts and cells critical for limb regeneration.

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Single-cell level transcriptomic anaylsis of the homeostatic and regenerating axolotl limb

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