The two-component system ChvGI responds to osmotic upshift and peptidoglycan damage in Caulobacter crescentus [ChIP-seq]
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ABSTRACT:
PROVIDER: PRJNA824713 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR18684852_1.fastq.gz | Fastqsanger.gz | |||
| SRR18684852_2.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:The two-component system ChvGI responds to osmotic upshift and peptidoglycan damage in Caulobacter crescentus.
| PRJNA824707 | ENA
Project description:Two-component systems (TCS) are often used by bacteria to rapidly assess and respond to environmental changes. ChvG/ChvI (ChvGI) is a TCS conserved in γ-proteobacteria and is known for regulating expression of genes related to exopolysaccharide production, virulence and growth. The sensor kinase ChvG autophosphorylates upon yet unknown signals and phosphorylates the response regulator ChvI to activate transcription. Recent studies in Caulobacter crescentus showed that chv mutants are sensitive to vancomycin treatment and fail to grow in synthetic minimal media. In this work, we identified the osmotic imbalance as the main cause of growth impairment in synthetic minimal media. We also determined the ChvI regulon and confirmed that ChvI regulates cell envelope architecture at different levels by controlling outer membrane, peptidoglycan assembly/recycling and inner membrane proteins. Furthermore, we identified genes with osmoregulatory properties and confirmed that osmotic upshift is a signal triggering ChvG-dependent phosphorylation of ChvI. In addition, we challenged chv mutants with other cell envelope related stress and found that targeting with antibiotics the transpeptidation of peptidoglycan during cell elongation impairs growth of the mutant. Moreover, these antibiotics activate expression of the chvIG-hprK operon in ChvI-dependent and independent ways. ChvI phosphorylation is also shown to be activated upon antibiotic treatment with vancomycin. Finally, we observed that the sensor kinase ChvG fused to a fluorescent protein relocates from a patchy-spotty distribution to distinctive foci after transition from complex to synthetic minimal media. Interestingly, this pattern of (re)location has been described for proteins involved in cell growth control and peptidoglycan synthesis upon osmotic shock. Overall, our data support that the ChvGI TCS is mainly used to monitor and respond to osmotic imbalances and damages in the peptidoglycan layer.
2022-04-11 | GSE200465 | GEO
Project description:Two-component systems (TCS) are often used by bacteria to rapidly assess and respond to environmental changes. ChvG/ChvI (ChvGI) is a TCS conserved in γ-proteobacteria and is known for regulating expression of genes related to exopolysaccharide production, virulence and growth. The sensor kinase ChvG autophosphorylates upon yet unknown signals and phosphorylates the response regulator ChvI to activate transcription. Recent studies in Caulobacter crescentus showed that chv mutants are sensitive to vancomycin treatment and fail to grow in synthetic minimal media. In this work, we identified the osmotic imbalance as the main cause of growth impairment in synthetic minimal media. We also determined the ChvI regulon and confirmed that ChvI regulates cell envelope architecture at different levels by controlling outer membrane, peptidoglycan assembly/recycling and inner membrane proteins. Furthermore, we identified genes with osmoregulatory properties and confirmed that osmotic upshift is a signal triggering ChvG-dependent phosphorylation of ChvI. In addition, we challenged chv mutants with other cell envelope related stress and found that targeting with antibiotics the transpeptidation of peptidoglycan during cell elongation impairs growth of the mutant. Moreover, these antibiotics activate expression of the chvIG-hprK operon in ChvI-dependent and independent ways. ChvI phosphorylation is also shown to be activated upon antibiotic treatment with vancomycin. Finally, we observed that the sensor kinase ChvG fused to a fluorescent protein relocates from a patchy-spotty distribution to distinctive foci after transition from complex to synthetic minimal media. Interestingly, this pattern of (re)location has been described for proteins involved in cell growth control and peptidoglycan synthesis upon osmotic shock. Overall, our data support that the ChvGI TCS is mainly used to monitor and respond to osmotic imbalances and damages in the peptidoglycan layer.
2022-04-11 | GSE200464 | GEO
Project description:The two-component system ChvGI responds to osmotic upshift and peptidoglycan damage in Caulobacter crescentus [RNA-seq]
| PRJNA824712 | ENA
Project description:This SuperSeries is composed of the SubSeries listed below.
2022-04-11 | GSE200466 | GEO
Project description:Time course of the S. meliloti response to an osmotic upshift elicited by salt (300mM or 400mM) or sucrose (500mM or 700mM)
2006-08-17 | E-MEXP-785 | biostudies-arrayexpress
Project description:Most bacteria possess a peptidoglycan cell wall, which is continuously remodeled during cell growth and divi¬sion. The peptidoglycan (PG) fragments generated in this process are typically imported into the cell and recycles through the PG biosynthesis pathway. While the underlying pathways have been studied intens¬ively in gamma¬proteobacteria, knowledge of their presence and physiological roles in other bacterial line¬ages remains limited. Here, we comprehensively investigate PG recycling in the alphaproteo¬bacterial model organism Caulo¬bacter crescentus. We show that this species contains a func¬tional PG recycling pathway by char¬acterizing the activities of key enzymes both in vitro and in vivo. Our results reveal that PG recycling is critical for maintaining C. crescentus cell morphology and division and is dynamically regu¬lated to balance the flux of metabolic intermediates toward PG biosynthesis and central carbon metabol¬ism. Im¬portantly, defects in PG recyc¬ling strongly impair the intrinsic ampicillin resistance of C. crescentus without changing the activity of its β-lactamase BlaA, likely by limiting PG precursor biosynthesis and thereby increasing the sensitivity of the septal FtsW-FtsI com-plex to residual antibiotic molecules. These findings underscore the central role of PG recyc¬¬ling in bacterial fitness and suggest that inhibiting this process could provide a promising strategy to combat β-lactam-resistant pathogens.
2026-03-11 | PXD069004 | Pride
Project description:The role of the S. cerevisiae Yap4 transcription factor in the response to hyperosmolarity was investigated in two ways. Firstly, to assess the overall response of the yap4 deleted strain genome wide analysis upon a mild upshift in osmolarity was compared to the same response in the wt strain. Secondly, in order to identify genes whose osmo-expression is differentially regulated in the mutant strain (ie potential Yap4 target genes) the relative abundance of cDNAs of the wt and yap4 mutant strain obtained upon an osmotic upshift were directly compared by microarray analysis. Keywords: Mutant Stress Response
2006-06-15 | GSE5070 | GEO
Project description:Determine if cells in stationary-phase cultures respond to increased temperature. Yeast cells in stationary-phase cultures were exposed to temperature upshift (30C to 39C) with samples harvested every 30-minutes over 8 hours. All experimental samples are over an common reference. There are two replicates for each time point and six replicates of T0.
2010-06-30 | E-GEOD-3687 | biostudies-arrayexpress
Project description:Potassium (K+) is an essential physiological element determining membrane potential, intracellular pH, osmotic/turgor pressure, and protein synthesis in cells. Here we describe the regulation of potassium uptake systems in the oligotrophic alpha-proteobacterium Caulobacter crescentus known as a model for asymmetric cell division. We show that C. crescentus can grow in concentrations from the micromolar to the millimolar range by mainly using two K+ transporters to maintain potassium homeostasis, the low affinity Kup and the high affinity Kdp uptake systems. When K+ is not limiting, we found that the kup gene is essential while kdp inactivation does not impact the growth. In contrast, kdp becomes critical but not essential and kup dispensable for growth in K+-limited environments. However, in the absence of kdp, mutations in kup were selected to improve growth in K+-depleted conditions, likely by increasing the affinity of Kup for K+. In addition, mutations in the KdpDE two-component system, which regulates kdpABCDE expression, suggest that the inner membrane sensor regulatory component KdpD mainly works as a phosphatase to limit the growth when cells reach late exponential phase. Our data therefore suggest that KdpE is phosphorylated by another non-cognate histidine kinase. On top of this, we determined the KdpE-dependent and independent K+ transcriptome. Together, our work illustrates how an oligotrophic bacterium responds to fluctuation in K+ availability.
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