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Long non-coding RNA GDAR regulate ovine granulosa cells apoptosis by affecting the expression of apoptosis-related genes

ABSTRACT: long non-coding RNA GDAR regulate ovine granulosa cells apoptosis by affecting the expression of apoptosis-related genes

PROVIDER: PRJNA825818 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18733166_1.fastq.gz	Fastqsanger.gz
	SRR18733166_2.fastq.gz	Fastqsanger.gz
	SRR18733167_1.fastq.gz	Fastqsanger.gz
	SRR18733167_2.fastq.gz	Fastqsanger.gz
	SRR18733168_1.fastq.gz	Fastqsanger.gz

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long non-coding RNA GDAR regulate ovine granulosa cells apoptosis by affecting the expression of apoptosis-related genes

Project description:Short-term dietary supplementation of female sheep during the luteal phase can increase fertility and most probably by stimulating glucose uptake by the follicles. However, the molecular mechanism of glucose regulates follicular development has not yet been clarified, especially the further study of long non-coding RNA (lncRNA) in determining fertility during follicular development. We generated GCs models of different doses of glucose (0, 2.1, 4.2, 8.4, 16.8 and 33.6 mM), and observed that the highest cell viability was recorded in the 8.4 mM group and the highest apoptosis rates were recorded in the 33.6 mM groups. Therefore, the control (n = 3, 0 mM glucose group), low glucose (n = 3, add 8.4 mM glucose concentration group), and high glucose (n = 3, add 33.6 mM glucose concentration group) of GCs were created for the next whole genomic RNA sequencing (RNA-seq). In total, 11,221 novel lncRNAs and 510 annotated lncRNAs were identified in the GCs samples. Gene ontology term enrichment indicates the obvious enrichment in apoptosis and its related pathways, which suggests that cell apoptosis could play a critical role in the process of glucose-induced GCs differentiation. Furthermore, we focused on the function of a lncGDAR and verified that lncGDAR could involve cell apoptosis by effecting the expression of Bcl-2, BAX, Caspase-3, and Caspase-7 genes. These results provide the basis for further study of LncRNA regulation mechanism in nutrition on female fertility.

2022-05-18 | GSE200668 | GEO

Long Non-Coding RNA <i>GDAR</i> Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related Genes.

Project description:Short-term dietary supplementation of ewes during the luteal phase can increase fertility, most probably by stimulating glucose uptake by the follicles. However, the molecular mechanism of glucose regulation of follicular development has not yet been clarified, especially the further study of long non-coding RNA (lncRNA) in determining fertility during follicular development. We generated granulosa cell (GC) models of different doses of glucose (0, 2.1, 4.2, 8.4, 16.8 and 33.6 mM), and observed that the highest cell viability was recorded in the 8.4 mM group and the highest apoptosis rates were recorded in the 33.6 mM group. Therefore, a control group (n = 3, 0 mM glucose), a low glucose group (n = 3, add 8.4 mM glucose), and a high glucose group (n = 3, add 33.6 mM glucose) of GCs were created for next whole genomic RNA sequencing. In total, 18,172 novel lncRNAs and 510 annotated lncRNAs were identified in the GCs samples. Gene Ontology indicated that differentially expressed lncRNAs associated with cell apoptosis were highly enriched. Kyoto Encyclopedia of Genes and Genomes enrichment analysis of lncRNA target genes found that the apoptosis pathway and the p53 signaling pathway were both enriched. Furthermore, we focused on the function of a lncGDAR and verified that lncGDAR could influence cell apoptosis in GC development through affecting the mRNA and protein expression of apoptosis-related markers. These results provide the basis for further study of the lncRNA regulation mechanism in nutrition on female fertility.

| S-EPMC9104640 | biostudies-literature

Prolactin Regulates Ovine Ovarian Granulosa Cell Apoptosis by Affecting the Expression of <i>MAPK12</i> Gene.

Project description:Prolactin (PRL) has been reported to influence reproductive performance and cell apoptosis. However, its mechanism remains unclear. Hence, in the present study, ovine ovarian granulosa cells (GCs) were used as a cell model to investigate the relationship between PRL concentration and GC apoptosis, as well as its possible mechanisms. We examined the relationship between serum PRL concentration and follicle counts in sexually mature ewes. GCs were isolated from adult ewes and treated with different concentrations of PRL, while 500 ng/mL PRL was selected as the high concentration of prolactin (HPC). Then, we applied the transcriptome sequencing (RNA-Seq) combined with a gene editing approach to explore the HPC contributing to cell apoptosis and steroid hormones. The apoptosis of GCs gradually increased at PRL concentrations above 20 ng/mL, while 500 ng/mL PRL significantly decreased the secretion of steroid hormones and the expression of L-PRLR and S-PRLR. The results indicated that PRL regulates GC development and steroid hormones mainly through the target gene MAPK12. The expression of MAPK12 was increased after knocked-down L-PRLR and S-PRLR, while it decreased after overexpressed L-PRLR and S-PRLR. Cell apoptosis was inhibited and the secretion of steroid hormones increased after interfering with MAPK12, while the overexpression of MAPK12 showed the opposite trend. Overall, the number of follicles gradually decreased with increasing PRL concentration. HPCs promoted apoptosis and inhibited steroid hormone secretion in GCs by upregulating MAPK12 through reducing L-PRLR and S-PRLR.

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Multi-Omics Analysis of Ovine Atretic Follicles：Linoleic Acid Metabolite 13-Hydroxyoctadecadienoic Acid as a Biphasic Ferroptosis Modulator in Granulosa Cells

Project description:Follicular atresia is a complex physiological process involving multiple forms of cell death. Ferroptosis, an iron-dependent form of programmed cell death, has been less studied in the context of follicular atresia. 13-Hydroxyoctadecadienoic acid (13(S)-HODE) is a bioactive lipid derived from linoleic acid. To investigate the association between ovine follicular atresia and ferroptosis, we performed transcriptomic and metabolomic analyses of healthy and atretic sheep follicles. The metabolomic analysis identified 87 and 48 differentially expressed metabolites in healthy and atretic follicles, respectively. Functional enrichment of atretic follicle fluid highlighted pathways related to linoleic acid and purine metabolism. Transcriptomic analysis revealed 250 highly expressed genes in ovarian granulosa cells of atretic follicles. Enrichment analysis indicated that these differentially expressed genes were associated with fatty acid metabolism and ferroptosis. Integration of multi-omics data demonstrated the occurrence of ferroptosis in atretic follicles, where 13(S)-HODE drives granulosa cell ferroptosis via the linoleic acid metabolism pathway

2025-03-28 | GSE292797 | GEO

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Project description:Ovine Metagenome

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FOXO1/3 and PTEN Depletion in Granulosa Cells Promotes Ovarian Granulosa Cell Tumor Development

Project description:The Forkhead Box, FOXO1 and FOXO3, transcription factors regulate multiple functions in mammalian cells. Selective inactivation of the Foxo1 and Foxo3 genes in murine ovarian granulosa cells severely impairs follicular development and apoptosis causing infertility, and as shown herein, granulosa cell tumor (GCT) formation. Coordinate depletion of the tumor suppressor Pten gene in the Foxo1/3 strain enhanced the penetrance and onset of GCT formation A direct comparison of ovarian granulosa cells from wild type d25 and FOXO/PTEN knockout granulosa cell tumors.

2015-06-17 | E-GEOD-67662 | biostudies-arrayexpress

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FOXO1/3 and PTEN Depletion in Granulosa Cells Promotes Ovarian Granulosa Cell Tumor Development

2015-06-17 | GSE67662 | GEO

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Project description:Ovine adenovirus A overview

| PRJNA69583 | ENA

H3K27me3 modulates aberrant transcription and apoptosis in cyclophosphamide-induced ovarian granulosa cell injury

Project description:H3K27me3 modulates aberrant transcription and apoptosis in cyclophosphamide-induced ovarian granulosa cell injury

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