Project description:To determine the accessible chromatin regions of Chamaelirium luteum genome, we performed the Assay for Transposase Accessible Chromatin with high-throughput sequencing (ATAC-seq) to generate the genome-wide chromatin accessibility profile.
Project description:To determine the size and sequence composition of the exceptionally large centromeres in the genome of monocentric Chamaelirium luteum, we performed CENH3-ChIPseq using the customized species-specific CENH3 antibody. We mixed the chromatins of C. luteum and Secale cereal (inbred line Lo7) to dilute the highly abundant centromeric Chama satellite repeats (9.3%) in the C. luteum genome before immunoprecipitation.
Project description:To determine the size and sequence composition of the exceptionally large centromeres in the genome of monocentric Chamaelirium luteum, we performed CENH3-ChIPseq using the customized species-specific CENH3 antibody. We mixed the chromatins of C. luteum and Secale cereal (inbred line Lo7) to dilute the highly abundant centromeric Chama satellite repeats in the C. luteum genome before immunoprecipitation. In addition, H3K4me2- and H3K9me2-ChIPseq were performed to verify the large-scale eu- and heterochromatic genome organization.
