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Transcriptomes analyses of AARS1 knockdown HGC-27 cells treated with lactate

ABSTRACT: Transcriptomes analyses of AARS1 knockdown HGC-27 cells treated with lactate

PROVIDER: PRJNA826823 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18752212_1.fastq.gz	Fastqsanger.gz
	SRR18752212_2.fastq.gz	Fastqsanger.gz
	SRR18752213_1.fastq.gz	Fastqsanger.gz
	SRR18752213_2.fastq.gz	Fastqsanger.gz
	SRR18752214_1.fastq.gz	Fastqsanger.gz

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Transcriptomes analyses of AARS1 knockdown HGC-27 cells treated with lactate

Project description:To investigat the role of AARS1 in HGC27 cells treated with lactate, we transfected HGC27 cells with AARS1 siRNA and then treated the cells with 25mM lactate for 24h

2024-05-29 | GSE200789 | GEO

Transcriptomes analyses of gastric cancer HGC-27 cell line treated with lactate

Project description:Transcriptomes analyses of gastric cancer HGC-27 cell line treated with lactate

| PRJNA826822 | ENA

Whole transcriptomes analyses of gastric cancer HGC-27 cell line treated with SHAP or control peptide

Project description:Purpose: To explore the molecular mechanisms of SHAP peptide (also termed as SOS) regulating gastic cancer tumorigenesis, RNA sequencing was performed to analyze the geome-wide change of SHAP treated HGC-27 cells compared to those of control peptide cells. Methods: Total mRNA was extracted from HGC-27 cells. Then RNA quality was assessed using an Agilent Bioanalyzer 2100 and the sample reads were sequenced using Illumina Hiseq 4000 platform. As a reasult, we got the transcript data using Hisat2 followed by Stringtie. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Results: We mapped about 60 million sequence reads per sample to the human genome and identified about 58,000 transcripts in HGC-27 cells. Apprioximately 1,000 transcripts showed different expression between siSTRN3 and n.c. HGC-27 cells, with a fold change ≥2 and p value <0.05. Gene set enrichment analysis (GSEA) showed a significantly negative enrichment of Hippo target genes. Conclusion: Our study present the datiled transcripts analysis of HGC-27 cells, with biologic replicates. Based on RNA-seq transcriptome characterization , we conclude a molecular mechanism of SHAP regulating Hippo pathway in gastric cancer.

2020-06-05 | GSE135086 | GEO

Transcriptomes analyses of gastric cancer HGC-27 cell line treated with lactate

Project description:Lactate is a major metabolite of tumor cell as a result of Warburg effect. Yet it remains elusive whether and how intracellular lactate may ever drive tumor cell proliferation. To investigat the role of lactate in proliferating cancer cells, we treated HGC27 cells with lactate and then sent for RNA-sequencing analysis.

2024-05-29 | GSE200790 | GEO

Whole transcriptomes analyses of gastric cancer HGC-27 cell line treated with siSTRN3 mixture or negative control siRNA

Project description:Purpose: To explore the molecular mechanisms of STRN3 regulating gastic cancer tumorigenesis, RNA sequencing was performed to analyze the geome-wide change of siSTRN3 treated HGC-27 cells compared to those of control cells. Methods: Total mRNA was extracted from HGC-27 cells in triplicate respectively. Then RNA quality was assessed using an Agilent Bioanalyzer 2100 and the sample reads were sequenced using Illumina Hiseq 4000 platform. As a reasult, we got the transcript data using Hisat2 followed by Stringtie. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Results: We mapped about 60 million sequence reads per sample to the human genome and identified about 58,000 transcripts in HGC-27 cells. Apprioximately 1,000 transcripts showed different expression between siSTRN3 and n.c. HGC-27 cells, with a fold change ≥2 and p value <0.05. Gene set enrichment analysis (GSEA) showed a significantly negative enrichment of Hippo target genes. Conclusion: Our study present the datiled transcripts analysis of HGC-27 cells, with biologic replicates. Based on RNA-seq transcriptome characterization , we conclude a molecular mechanism of STRN3 regulating Hippo pathway in gastric cancer.

2020-06-05 | GSE135085 | GEO

Whole transcriptomes analyses of HGC-27 cell line treated with control DMSO, disulfiram, thiram or CX-6258

Project description:Purpose: To figure out the molecular function of disulfiram, thiram and CX-6258, RNA sequencing was performed to analyze the geome-wide change of disulfiram, thiram and CX-6258 treated HGC-27 cells compared to those of control DMSO treated cells. Methods: Total mRNA was extracted from HGC-27 cells. Then RNA quality was assessed using an Agilent Bioanalyzer 2100 and the sample reads were sequenced using Illumina Hiseq 4000 platform. As a reasult, we got the transcript data using Hisat2 followed by Stringtie. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Conclusion: The results showed disulfiram, thiram and CX-6258 may target distinct combinations of various pathways including Hippo pathway. The Hippo signaling indeed can be targeted by disulfiram, thiram and CX-6258 even though these compounds also interfere with other biological processes.

2024-06-12 | GSE168618 | GEO

Transcriptomic changes associated with GPNMB knockdown in HGC-27 gastric cancer cells

Project description:This study characterized transcriptomic changes associated with GPNMB knockdown in HGC-27 gastric cancer cells. Cells were transiently transfected with either GPNMB-targeting siRNA or a non-targeting control siRNA. RNA sequencing was performed using three independently prepared biological samples per group. Differential-expression and enrichment analyses were used to identify candidate biological processes and signaling pathways associated with GPNMB knockdown. Several pathways, including cAMP signaling, appeared among the nominal enrichment outputs. The cAMP pathway was therefore treated as a hypothesis-generating candidate for subsequent experimental investigation. The deposited transcriptomic dataset does not by itself establish an exclusive downstream signaling pathway, immune-suppressive function, clinical prognostic value, or therapeutic efficacy.

2026-05-17 | GSE330529 | GEO

Transcriptomic changes associated with GPNMB knockdown in HGC-27 gastric cancer cells

Project description:Transcriptomic changes associated with GPNMB knockdown in HGC-27 gastric cancer cells

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Transcriptome profiling of over-expression of GC0643 in HGC-27 cancer cells

Project description:To investigate the molecular biological function of GC0643, we enforced GC0643 overexpression in HGC-27 cell lines.

2022-10-12 | GSE207820 | GEO

Transcriptome analysis of HGC-27 after infection with Sendai virus

Project description:To better understand the interactions between Hippo signaling and antiviral signaling at transcription level, a high-throughput RNA-seq technology was utilized in gastric cancer cells HGC-27 at 48 hours after Sendai virus (SeV) infection.

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