Project description:Charme is a murine long non-coding RNA necessary for the embryonic development of the heart. In vivo Charme knock-out causes prominent alterations of tissue structure, due to cardiac hyperplasia, and leads to the development of cardiac dysfunctions. Although its role in cardiomyocytes has been deeply investigated, no evidence has been collected about its function in other cardiac cell types. Cardiac fibroblasts (CFs) play pivotal roles in the development and homeostatic maintenance of the cardiac tissue. This study investigated the phenotype and function of resident CFs isolated from Charme knockout (CharmeKO) mice, and revealed impaired maturation and functionality. CharmeKO hearts show decreased levels of collagen I content in the extracellular matrix, associated with reduced extracellular matrix-related gene expression and matrix remodelling ability of the CFs. CharmeKO CFs show impaired phenotypic conversion into myofibroblasts and reduced responsiveness to activation stimuli, accompanied by the retention of features proper of unactivated mesenchymal cells. Charme depletion also affects CF paracrine function, determining an impoverishment in cardioprotective cytokines in their secretome, with consequent reduced ability to mediate PI3K/Akt pathway activation in cardiomyocytes and to induce the angiogenic process in endothelial cells. CharmeKO CFs also proved to be less supportive to cardiomyocyte maturation in an in vitro model of cardiac differentiation, thus indicating a potential contribution to the in vivo impairment of cardiomyocyte maturation. Overall, the evidence collected suggests that Charme depletion in the heart impacts CF maturation capacity, leading to their impaired function, which can potentially contribute to the alterations observed in the CharmeKO mice. These findings pave the way to deeper investigations of the intercellular signalling occurring in the heart upon Charme ablation with the aim of identifying how microenvironment homeostasis contributes to cardiomyocyte maturation, and is potentially involved in cardiac diseases.

			Action	DRS
	SRR18769746_1.fastq.gz	Fastqsanger.gz
	SRR18769746_2.fastq.gz	Fastqsanger.gz
	SRR18769747_1.fastq.gz	Fastqsanger.gz
	SRR18769747_2.fastq.gz	Fastqsanger.gz
	SRR18769748_1.fastq.gz	Fastqsanger.gz

Dataset Information

Effect of Charme lncRNA knockout (KO) in cardiac development [RNA-seq]

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