Project description:Black cumin (Nigella sativa L.) is known to possess a wide variety of antimicrobial peptides belonging to different structural families. Three novel antimicrobial peptides have been isolated from black cumin seeds. Two of them were attributed as members of the non-specific lipid transfer proteins family and one - as a defensin. We have made an attempt of using proteomic approach for novel antimicrobial peptides search in N. sativa seeds as well. The use of well established approach that includes extraction and fractionation stages remains relevant even in case of novel peptides search because the lacking of N. sativa genome data. Novel peptides demonstrate a spectrum of antimicrobial activity against plant pathogenic organisms that may cause economically important crop diseases. These results obtained allow considering these molecules as candidates to be applied in "next-generation" biopesticides development for agriculture use.
Project description:To evaluate the roles of gene regulation in Oryza sativa leaf, dynamic profiles of transcriptome were investigated in Oryza sativa L. spp. indica with different treatments, the aerial tissues of one-month-old plants from four different areas (groups 1–4) were treated with 0, 40 mL of 25% azoxystrobin, 0.01 g of VdAL, or 40 mL of 25% azoxystrobin plus 0.01 g VdAL, respectively.
Project description:Time course: Interaction of Magnaporthe isolate TH6772 (of the host plant rice) with Hordeum vulgare, Ingrid (leaf epidermis) and Magnaporthe isolate CD180 (of Pennisetum) with Hordeum vulgare, Ingrid (leaf epidermis)
