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Transcriptomes of Wild Type tomato and slszp1 under +Al treatment

ABSTRACT: Transcriptomes of Wild Type tomato and slszp1 under +Al treatment

PROVIDER: PRJNA828447 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18831527_1.fastq.gz	Fastqsanger.gz
	SRR18831527_2.fastq.gz	Fastqsanger.gz
	SRR18831528_1.fastq.gz	Fastqsanger.gz
	SRR18831528_2.fastq.gz	Fastqsanger.gz
	SRR18831529_1.fastq.gz	Fastqsanger.gz

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Transcriptomes of Wild Type tomato and slstop1 under -Al/+Al treatment

Project description:Transcriptomes of Wild Type tomato and slstop1 under -Al/+Al treatment

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Transcriptomes of Wild Type tomato and slstop1 under -Al/+Al treatment

Project description:Purpose: Find SlSTOP1 regulated genes Methods: Total RNA was extracted from 20 days old wild-type tomato and slstop1 mutant plants treated with 0 and 60 μM Al solutions (pH 4.7). Three biological replicated samples were collected form each treatment and sequenced. Results:A total of 83.7 GB clean data was obtained by transcriptome sequencing. The clean data of each sample was more than 6.30 GB, and the percentage of q30 base was more than 93.82%. Conclusions: By RNA-seq analysis, we find potential SlSTOP1-regulated genes

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Transcriptomes of Wild Type tomato and slszp1 under +Al treatment

Project description:Purpose: Find SlSZP1 regulated genes Methods: Total RNA was extracted from 20 days old wild-type tomato and slszp1 mutant plants treated with 60 μM Al solutions (pH 4.7). Three biological replicated samples were collected form each treatment and sequenced. Results:A total of 40 GB clean data was obtained by transcriptome sequencing. The clean data of each sample was more than 6.11 GB, and the percentage of q30 base was more than 90.98%. Conclusions: By RNA-seq analysis, we find potential SlSZP1-regulated genes

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Tomato root culture and Al treatment

Project description:cDNA microarrays (Tom 1) were used to analyze changes in transcript accumulation in tomato (Solanum lycopersicum cv Money Maker) root cultures under Al-stress (20uM AlK(SO4)2). Out of the 1,362 genes that had significant changes in transcript accumulation (FDR<0.05), 569 were reduced and 793 were induced. The down-regulated genes included those found in the cell cycle (p34cdc2 protein kinase, cyclin, histone), putriscine synthesis, ABC transporter, germin-like protein. Al-induced genes included glutamate synthase, pectin modification, histidine synthesis, proline synthesis, metallothionein-like protein, multidrug efflux proteins (MATE), ethylene biosynthesis and detoxification proteins. Different isoforms in the multiple gene families of V-ATPase, glutathione transferases and others were either induced or repressed. A wide array of genes for regulating transcription and translation activities, and signal transduction were also affected.

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Transcriptomes of Wild Type tomato and sllbd40 fruit on different fruit stages

Project description:In order to find the gene which were regulated by SlMYC2, the fruits of WT and SlMYC2-KO line were collected at 25 DDA to do the RNA-Seq. Total RNA was extracted from above tomato fruits by TRIzol reagent and three biological replicates were taken. The clean data were aligned to the tomato reference genome release SL3.0. According to the RNA-Seq data, many development-related genes were down-regulated in SlMYC2-KO fruit. We find the potential SlMYC2-regulated genes.

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Project description:In order to find the gene which were regulated by SlLBD40, the fruits of WT and SlLBD40-KO-5 line were collected at 25 DDA to do the RNA-Seq. Total RNA was extracted from above tomato fruits by TRIzol reagent and three biological replicates were taken. The clean data were aligned to the tomato reference genome release SL2.50. According to the RNA-Seq data, many development-related genes were down-regulated in SlLBD40-KO-5 fruit. We find the potential SlLBD40-regulated genes.

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2012-02-27 | E-GEOD-29171 | biostudies-arrayexpress

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Project description:Transcriptomes of Wild Type tomato and sllbd40 fruit on different fruit stages

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