Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Biologic characteristics of B-lymphoblastic leukemia correspond to immunoglobulin gene diversity [array]

ABSTRACT: Biologic characteristics of B-lymphoblastic leukemia correspond to immunoglobulin gene diversity [array]

PROVIDER: PRJNA828590 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Biologic characteristics of B-lymphoblastic leukemia correspond to immunoglobulin gene diversity

Project description:Biologic characteristics of B-lymphoblastic leukemia correspond to immunoglobulin gene diversity

| PRJNA828588 | ENA

Biologic characteristics of B-lymphoblastic leukemia correspond to immunoglobulin gene diversity [sequencing]

Project description:Biologic characteristics of B-lymphoblastic leukemia correspond to immunoglobulin gene diversity [sequencing]

| PRJNA825267 | ENA

Biologic characteristics of B-lymphoblastic leukemia correspond to immunoglobulin gene diversity [sequencing]

Project description:It remains unclear whether the variable biologic features of B-lymphoblastic leukemia (B-ALL) reflect distinct cells of origin in the hierarchy of early B cell development. Recombination activating genes (RAG) 1 and 2 rearrange the immunoglobulin heavy chain (IgH) locus in B lymphoid progenitors, resulting in unique variable (Vh), diversity (D), and joining (Jh) gene rearrangements. To test whether discrete properties of each B-ALL reflect derivation from distinct developmental B cell stages, we assessed the relationship between IgH variable gene (IGHV) characteristics and gene expression. Using targeted, pre-treatment IgH sequencing of 22 patients’ B-ALL, we discovered 2 distinct groups: leukemias with RAG-mediated intra-clonal IGHV sequence diversification (‘diverse’; N=9, 40.9%) and those lacking diversity (‘homogeneous’; N=10, 45.5%). Three patients (13.6%) lacked a dominant IgH sequence. In the ‘diverse’ cohort, we observed extensive subclone evolution arising from Vh gene recombination after neoplastic transformation (median: 482 subclones; range 2-2600). In the ‘homogeneous’ cohort, all IgH clones lacked subclone evolution. The ‘diverse’ cohort demonstrated enriched expression of genes associated with regulation of a hematopoietic stem cell state, while ‘homogeneous’ cases showed enriched expression of genes involving cell fate commitment. Furthermore, distinct IgH clonotypes within a single patient could be individually diverse or homogenous. Single cell analysis indicated that within a single patient, only occasional IgH clonotypes were expressed; IGHV-expressing leukemia cells were characterized by distinctive phenotypes including RAG1 pathway up-expression. In summary, pre-treatment IgH composition reflects the B cell stage at leukemic initiation and provides insights about the biologic mechanisms of genetic diversity in B-ALL.

2025-04-08 | GSE200557 | GEO

Biologic characteristics of B-lymphoblastic leukemia correspond to immunoglobulin gene diversity [array]

Project description:We tested the relationship between IgH subclone diversity in B-ALL and the number of resolved chromosomal breaks as assessed by chromosome microarray analysis (CMA).

2025-04-08 | GSE201170 | GEO

Biologic characteristics of B-lymphoblastic leukemia correspond to immunoglobulin gene diversity

Project description:This SuperSeries is composed of the SubSeries listed below.

2025-04-08 | GSE201171 | GEO

Expression data from Down syndrome and non-Down syndrome pediatric acute lymphoblastic leukemia cases

Project description:Gene expression profiling (GEP) can reveal characteristic signatures associated with distinct biologic subtypes of acute lymphoblastic leukemia (ALL). We performed GEP on Down syndrome (DS) and comparison non-Down syndrome (NDS) ALL cases to identify biologic differences between these groups. Ficoll-enriched, cryopreserved diagnostic bone marrow samples were obtained from patients with newly diagnosed B-precursor acute lymphoblastic leukemia.

2011-07-12 | E-GEOD-20910 | biostudies-arrayexpress

Expression data from Down syndrome and non-Down syndrome pediatric acute lymphoblastic leukemia cases

2011-07-12 | GSE20910 | GEO

Methylation data from Down syndrome and non-Down syndrome pediatric acute lymphoblastic leukemia cases and controls

2011-12-01 | GSE20872 | GEO

SNP data from Down syndrome and non-Down syndrome pediatric acute lymphoblastic leukemia cases and controls

Project description:SNP profiling can reveal copy number abnormalities and loss of heterozygosity associated with distinct biologic subtypes of acute lymphoblastic leukemia (ALL). We performed SNP profiling of Down syndrome ALL cases and controls to identify unique biologic features of this ALL subgroup.

2011-07-12 | GSE21091 | GEO

Methylation data from Down syndrome and non-Down syndrome pediatric acute lymphoblastic leukemia cases and controls

Project description:Methylation profiling can reveal patterns of hypermethlation and hypomethylation associated with distinct biologic subtypes of acute lymphoblastic leukemia (ALL). We performed methylation profiling of Down syndrome ALL cases and controls to identify unique biologic features of this ALL subgroup. Ficoll-enriched, cryopreserved bone marrow aspirate samples were obtained from patients with B-precursor ALL at diagnosis and in remission

2011-12-01 | E-GEOD-20872 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data