Project description:RNA-sequencing polyA library preparation in wing discs (WD) and pupal wings (PW) from inbred (D907) and outbred (Mix) fly populations from lines selected for the ectopic veins trait under genetic assimilation (assimilated) or direct artificial selection under normal conditions (non-assimilated) and unselected (control) flies.
Project description:Sequencing was performed to assess the ability of Nanopore direct cDNA and native RNA sequencing to characterise human transcriptomes. Total RNA was extracted from either HAP1 or HEK293 cells, and the polyA+ fraction isolated using oligodT dynabeads. Libraries were prepared using Oxford Nanopore Technologies (ONT) kits according to manufacturers instructions. Samples were then sequenced on ONT R9.4 flow cells to generate fast5 raw reads in the ONT MinKNOW software. Fast5 reads were then base-called using the ONT Albacore software to generate Fastq reads.
Project description:OnT dRNA of K562 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:ONT dRNA of HepG2 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:RNA-seq of porcine peripheral whole blood from pig divergently selected for residual feed intake and treated with lipopolysaccharide
