Project description:Microbial fermentation is involved in the processing of a dark tea popular for centuries in Northwest China which has shown many health benefits. This study will examine anti-obesity, hyperlipidemic and hyperglycemic effects of CGMCC No.8730 Eurotium cristatum (EC) fermented dark tea (8730DT).
Project description:In the present study, we studied microbial composition and metabolic activity in the bathypelagic zone of the South China Sea. 12 samples were collected and subjected to metaproteomic analysis. Our data provide a novel view of the roles of two lifestyle prokaryotes and their link in substrate utilization in dark ocean.
Project description:This study evaluated glycine as a sole carbon source in an EBPR sequencing batch reactor, demonstrating effective phosphorus removal comparable to systems fed with mixed substrates. Microbial and genome-resolved analyses revealed a community with complementary metabolic roles, highlighting its contribution to phosphorus removal dynamics.
Project description:Metabolic diseases, such as obesity, diabetes, and cardiovascular diseases, pose significant health challenges in contemporary society. The development of these diseases is closely related to the balance of gut microbiota, the functionality of metabolic products, and metabolic dysregulation across various tissues and organs. Tibetan tea, a traditional post-fermented tea originating from Ya'an, Sichuan in Southwest China, is known for its unique fermentation process and flavor, as well as its multitude of health benefits, such as acting as a prebiotic, antioxidant, blood sugar reducer, antithrombotic, and obesity prevention agent. This has sparked widespread interest among scholars both domestically and internationally in its potential to address metabolic diseases such as obesity, diabetes, hyperlipidemia, and thrombosis formation. However, current research has mostly focused on the effects on single tissues, with less exploration into the role of Tibetan tea in multi-tissue responses. Therefore, this study employed large-scale sequencing technologies to deeply analyze the impact of Tibetan tea extract intervention on the gut microbiota and peripheral blood, as well as on the metabolism of 10 different tissues and organs, including the brain, brown fat, heart, liver, kidneys, pancreas, colon, white fat, skeletal muscle, and bone marrow in C57BL/6J mice under both normal and high-fat diet conditions. The aim was to reveal the holistic response and mechanism of host metabolism to Tibetan tea intervention, thereby providing new insights into nutritional intervention strategies for metabolic diseases.
Project description:Pu-erh tea has attracted increasing attention worldwide because of its special flavor and health effects, but its impact on composition and function of the gut microbiota remains unclear. The aim of this study was to investigate effects of aqueous extracts of fermented (ripe) and non-fermented (raw) Pu-erh teas on the composition and function of intestinal microbiota of rats with diet-induced obesity. We conducted a comparative metagenomic and metaproteomic investigation of the microbial communities in cecal samples taken from obese rats administrated with or without extracts of raw and ripe Pu-erh tea. By analyzing the composition and diversity of 16S rRNA amplicons and expression profiles of 814 distinct proteins, we found that, despite differences in the chemical compositions of the raw and ripe Pu-erh tea, administration of either at two different doses (0.15 and 0.40 g/Kg body weight), significantly (P<0.05) increased community diversity, and changed the composition of the cecal microbiota by increasing the relative abundances of Firmicutes and decreasing those of Bacteroidetes. Community metabolic processes including sucrose metabolism, glycolysis, syntheses of proteins, rRNA and antibiotics were significantly (P<0.05), or had a tendency (0.10<P<0.05) to be, promoted by enriching relevant enzymes. Furthermore, evidences from population, molecular and metabolic levels have shown that polyphenols of raw Pu-erh tea and their metabolites can promote potentially the growth of Akkermansia municiphila by stimulating the type II and III secretion system protein, elongation factor Tu, and glyceraldehyde-3-phosphate dehydrogenase. This study has provided new evidences for the prebiotic effects of Pu-erh tea.
Project description:Global warming has shifted climate zones poleward or upward. However, understanding the responses and mechanism of microbial community structure and functions relevant to natural climate zone succession is challenged by the high complexity of microbial communities. Here, we examined soil microbial community in three broadleaved forests located in the Wulu Mountain (WLM, temperate climate), Funiu Mountain (FNM, at the border of temperate and subtropical climate zones), or Shennongjia Mountain (SNJ, subtropical climate).Soils were characterized for geochemistry, Illumina sequencing was used to determine microbial taxonomic communities and GeoChips 5.0 were used to determine microbial functional genes.