Project description:Innate immune cells can acquire a memory phenotype, termed trained immunity, but the mechanism underlying the regulation of trained immunity remains largely elusive. Here, we demonstrate that inhibition of Aurora kinase A (AurA) dampens trained immunity induced by β-glucan. ATAC-seq and RNA-seq analysis reveals that AurA inhibition restricts chromatin accessibility of genes associated with inflammatory pathways such as JAK-STAT, TNF and NF-κB pathways. Specifically, AurA inhibition promotes nuclear localization of FOXO3 and the expression of glycine N-methyltransferase (GNMT), a key enzyme responsible for adenosylmethionine (SAM) consumption. Metabolomic analysis confirms a reduction in SAM level upon AurA inhibition. As a result of SAM deficiency, trained mouse macrophages exhibit decreased H3K4me3 and H3K36me3 enrichment on gene regions of Il6 and Tnfα. Additionally, the tumor inhibition effect of β-glucan is notably abolished by AurA inhibition. Together, our findings identify an essential role of AurA in regulating trained immunity via a methylation-dependent manner by maintaining endogenous SAM level through mTOR-FOXO3-GNMT axis

2025-08-01 | GSE280935 | GEO

Project description:Cathartes melambrotus complete mitochondrial genome

| PRJNA1167675 | ENA

RNA profiling by deepSAGE sequencing in the cortex of a migraine mouse model after induction of cortical spreading depression

Project description:Familial Hemiplegic Migraine type 1 (FHM1) is a rare monogenic subtype of migraine with aura caused by mutations in CACNA1A that encodes the a1A subunit of voltage-gated CaV2.1 calcium channels. Transgenic knock-in mice that carry the human FHM1 R192Q missense mutation (“FHM1 R192Q mice”) exhibit an increased susceptibility to cortical spreading depression (CSD), the mechanism underlying migraine aura. Here we analysed gene expression profiles from isolated cortical tissue of FHM1 R192Q mice 24 hours after experimentally induced CSD in order to identify molecular pathways affected by CSD. Gene expression profiles were generated using deep Serial Analysis of Gene Expression sequencing. Our data reveal a signature of inflammatory signalling upon CSD in the cortex of both mutant and wild-type mice. However, only in the brains of FHM1 R192Q mice specific genes are up-regulated in response to CSD that are implicated in interferon-related inflammatory signalling. Our findings show that CSD modulates inflammatory processes in both wild-type and mutant brains, but that an additional unique inflammatory signature becomes expressed after CSD in a relevant mouse model of migraine.

2016-04-14 | GSE67933 | GEO

The complete mitochondrial genome and phylogenetic analyses of <i>Cathartes melambrotus</i> (Wetmore 1964) (Aves: Cathartidae).

Project description:Cathartes melambrotus is the largest member of the genus Cathartes, and soars over the forested areas of Amazonia in search of carrion. The complete mitochondrial genome of C. melambrotus was reported in this study. The 19,232 base pair genome consisted of 16 protein coding genes, 25 tRNAs, two rRNAs, and two control regions. The mitochondrial genome contained the avian ancestral duplicated gene region, with the same rearrangements previously reported in Accipitriformes, Cathartiformes, and Stigiformes. With the publishing of the C. melambrotus genome all seven Cathartiformes species mitochondrial genomes are available and can be included in subsequent phylogenetic and genomic analyses.

| S-EPMC11803758 | biostudies-literature

Calcarisporium arbuscula

Project description:Calcarisporium arbuscula and Calcarisporium arbuscula aurA Transcriptome

| PRJNA633469 | ENA