Dataset Information

Fecal microbiota transplantation from young donor mice rejuvenates hematopoietic stem cell in aged recipients by suppressing inflammatory signals while modulating gut microbiota and metabolites

ABSTRACT: Fecal microbiota transplantation from young donor mice rejuvenates hematopoietic stem cell in aged recipients by suppressing inflammatory signals while modulating gut microbiota and metabolites

PROVIDER: PRJNA833475 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	SRR18989600_1.fastq.gz	Fastqsanger.gz
	SRR18989600_2.fastq.gz	Fastqsanger.gz
	SRR18989601_1.fastq.gz	Fastqsanger.gz
	SRR18989601_2.fastq.gz	Fastqsanger.gz
	SRR18989602_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Project description:BACKGROUND: The gut-brain axis and the intestinal microbiota are emerging as key players in health and disease. Shifts in intestinal microbiota composition affect a variety of systems, however, evidence of their direct impact on cognitive functions is still lacking. We tested whether faecal microbiota transplant (FMT) from aged donor mice into young adult recipients affected the hippocampus, an area of the central nervous system (CNS) known to be affected by the ageing process, and related functions.METHODS AND FINDINGS: Young adult mice were transplanted with the microbiota from either aged or age-matched donor mice. Following transplantation, characterization of the microbiotas and metabolomics profiles along with a battery of cognitive and behavioural tests were performed. Label-free quantitative proteomics was employed to monitor protein expression in the hippocampus of the recipients. Gut permeability, levels of circulating cytokines and expression of markers of microglia cells were also assessed. FMT from aged donors led to impaired spatial learning and memory in young adult recipients, whereas anxiety, explorative behaviour and locomotor activity remained unaffected. This was paralleled by altered expression of proteins involved in synaptic plasticity and neurotransmission in the hippocampus. Also, a strong reduction of bacteria associated with short-chain fatty acids (SCFAs) production (Lachnospiraceae, Faecalibaculum and Ruminococcaceae) and disorders of the CNS (Prevotellaceae and Ruminococcaceae) was observed. Finally, microglia cells of the hippocampus fimbria, acquired an ageing-like phenotype, while gut permeability and levels of circulating cytokines remained unaffected.CONCLUSIONS: These results demonstrate a direct effect of the age-associated shifts of the microbiota on protein expression and key functions of the central nervous system. Furthermore, these results additionally highlight the paramount importance of the gut-brain axis in ageing and provide a strong rationale to devise therapies aiming to restore a young-like microbiota to improve cognitive functions in the elderly.

Dataset Information

Fecal microbiota transplantation from young donor mice rejuvenates hematopoietic stem cell in aged recipients by suppressing inflammatory signals while modulating gut microbiota and metabolites

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets