Project description:Heliophilum fasciatum is an anoxygenic photosynthetic bacteria isolated from rice soil. Previous work outlined the existence of five nitrogen-fixation-like gene clusters potentially involved in sulfur metabolism. In this study, cultures of H. fasciatum were grown with varying sulfate and thiosulfate concentrations, and the transcriptomes were generated using Illumina RNA-sequencing. Differential gene expression analysis was utilized to investigate sulfur-mediated regulation of these nitrogen-fixation-like systems and H. fasciatum genes.
Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
