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Reference Long and Short-Read Transcriptomes of Human Peripheral Blood Lymphocytes

ABSTRACT: Reference Long and Short-Read Transcriptomes of Human Peripheral Blood Lymphocytes

PROVIDER: PRJNA835471 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	SRR19100535_subreads.fastq.gz	Fastqsanger.gz
	SRR19100536_subreads.fastq.gz	Fastqsanger.gz
	SRR19100537_subreads.fastq.gz	Fastqsanger.gz
	SRR19100538_subreads.fastq.gz	Fastqsanger.gz
	SRR19100592.fastq.gz	Fastqsanger.gz

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LocusMasterTE: long-read assisted short-read TE quantification [short]

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Reference Long-Read Transcriptomes of Human Peripheral Blood Lymphocytes (PacBio Iso-Seq, Matched to Illumina RNA-Seq Data)

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LocusMasterTE: long-read assisted short-read TE quantification [short]

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Project description:Long-read sequencing technologies such as Iso-Seq (PacBio Inc.) generate highly accurate sequences of full-length mRNA transcript isoforms. Long-read transcriptomics may be especially useful in the context of T cell functional plasticity as it relates to human health and disease. However, To our knowledge, no long-read transcriptome reference exists for activated human CD4 T cells. To begin to fill this gap, we purified CD4 T cells from the peripheral blood of a healthy female donor and activated these cells with anti-CD3/CD28 beads to generate populations of early activated (4hr), mid-activated (16hr), blasting (48hr) and proliferating (120hr) CD4 T cells. From each of these time points, we obtained high-quality RNA (RIN>9) for PacBio Iso-Seq analysis and parallel RNA-Seq analysis, which we hope will serve as a reference for future transcriptomic studies of these populations. UCSC genome browser tracks for these samples can be accessed at: http://genome.ucsc.edu/cgi-bin/hgHubConnect?hgHub_do_redirect=on&hgHubConnect.remakeTrackHub=on&hgHub_do_firstDb=on&position=chr1:206,903,317-206,921,941&hubUrl=http://162.215.210.70/~tracks/Mitchell_IsoSeq_Stim/hub.txt

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