Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Low Dose Gamma Irradiation of Trypanosoma evansi Parasites Identifies Gene Transcripts Involved in Establishing Disease in Mice Post Irradiation

ABSTRACT: Low Dose Gamma Irradiation of Trypanosoma evansi Parasites Identifies Gene Transcripts Involved in Establishing Disease in Mice Post Irradiation

PROVIDER: PRJNA835481 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Low Dose Gamma Irradiation of Trypanosoma evansi Parasites Identifies Gene Transcripts Involved in Establishing Disease in Mice Post Irradiation

Project description:Transcriptome analysis of irradiated T evansi parasites The protozoan parasite Trypanosoma evansi is responsible for causing Surra in a variety of mammalian hosts over a wide geographical area. In order to identify which genes and processes are required to establish disease in mice, parasites were irradiated over a range using a Cobalt60 gamma source. A custom Trypanosome spp. array that covers the genomes of three trypanosome species, T. brucei, T. evansi and T. congolense was designed by Affymetrix with an average of 9300 whole gene transcripts from all three species were targeted. Irradiation differentially affected the abundance of gene transcripts in a dose-dependent trend. We present these genes as necessary for repair from irradiation damage, and essential for disease establishment in mice post irradiation.

2023-05-06 | GSE202334 | GEO

Low Dose Gamma Irradiation of Trypanosoma evansi Parasites Identifies Molecular Changes That Ooccur to Repair Radiation Damage and Gene Transcripts That May be Involved in Establishing Disease in Mice Post Irradiation

Project description:The protozoan parasite Trypanosoma evansi is responsible for causing Surra in a variety of mammalian hosts over a wide geographical area. In order to identify which genes and processes are required to establish disease in mice, parasites were irradiated over a range using a Cobalt60 gamma source. A custom Trypanosome spp. array that covers the genomes of three trypanosome species, T. brucei, T. evansi and T. congolense was designed by Affymetrix with an average of 9300 whole gene transcripts from all three species were targeted. Irradiation differentially affected the abundance of gene transcripts in a dose-dependent trend. We present these genes as necessary for repair from irradiation damage, and essential for disease establishment in mice post irradiation.

2022-05-10 | E-MTAB-11705 | biostudies-arrayexpress

RNA-seq of C57BL/6 mice splenocytes during Trypanosoma Evansi infection

Project description:We investigate changes in the single-cell transcriptomic profile of mouse (C57BL/6) splenocytes during Trypanosoma Evansi Merzoua-93 (ITMAS 150399C) infection. Infection was initiated by Intraperitoneal injection with parasites diluted in PBS. On day 0/14/42 post-injection, mice spleens were disassociated, RBC lysed to obtain the cells for 10x Chromium 3' Gene Expression Profiling

2021-10-26 | E-MTAB-10174 | biostudies-arrayexpress

Trypanosoma evansi

Project description:Trypanosoma evansi overview

| PRJNA1142666 | ENA

RNA-seq of C57BL/6 mice splenocytes at chronic stage of Trypanosoma evansi infection

Project description:We investigate changes in the single-cell transcriptomic profile of mouse (C57BL/6) splenocytes at late stage of Trypanosoma evansi Merzouga 93 (ITMAS 150399C) infection. Infection was initiated by Intraperitoneal injection with parasites diluted in PBS. On day 42 post-injection, mice spleens were disassociated, RBC lysed to obtain the cells for 10x Chromium 3' Gene Expression Profiling

2022-10-26 | E-MTAB-12109 | biostudies-arrayexpress

Trypanosoma evansi isolate:TEDC953

Project description:Trypanosoma evansi isolate:TEDC953 Genome sequencing

| PRJNA1142466 | ENA

Trypanosoma evansi secretome carries potential biomarkers for surra diagnosis

Project description:Trypanosoma evansi is a parasite of African origin that has evolutionary proximity to Trypanosoma brucei. This parasite, which causes the surra, has the broadest range of hosts, including wild and domestic mammals, and its geographic distribution is the widest among all trypanosomes. This disease causes numerous economic losses in livestock in countries in the Americas, Africa, and Asia. This work aims to obtain in vitro secreted proteins from T. evansi and identify potential treatment and diagnostic biomarkers of surra. For this, in an experimental group, the parasite was purified in an ion-exchange column (DEAE-Cellulose) and maintained in a secretion medium. In a second group, there was no purification of the parasites. Also, each group was maintained at 37 ºC and 27 ºC. Finally, we identified the proteins through mass spectrometry. We found a total of 246 proteins and noticed that the temperature appears to modulate protein secretion. We observed negligible differences in proteins from purified and non-purified sets. We observed that most secreted proteins are related to molecular function (F) of binding, catalytic activity, structural molecule activity, and biological process (P) of metabolic process, cellular process, localization, and biological regulation. We also verified that most secretome proteins have homologous proteins with trypanosomatids T. brucei, T. b. gambiense, T. vivax, and T. equiperdum. However, despite this, it was possible to identify the presence of exclusive T. evansi epitopes with potential biomarkers for differential surra diagnosis. We identified epitopes of proteins that are part of the glycolytic process of the parasite, which can be used in immunotherapy by the production of anti-proteins from the glycolytic process. Finally, this is the first work that provide a big picture of the T. evansi secretome and could be the starting point to elucidate biological issues related to surra.

2023-03-04 | PXD031861 | Pride

Landscapes of post-translational modifications in Trypanosoma parasites

Project description:Proteins of all living cells undergo a myriad of posttranslational modifications (PTMs) that are critical to multifarious life processes. In this study, we describe the first comprehensive multiple PTM-omics atlas in parallel with quantitative proteome analyses of two representative species of trypanosomes, Trypanosoma brucei and T. evansi. Approximately 40,000 modified sites and 150 histone marks were identified in each species. Proteins involved in the redox system were mainly regulated in T. brucei, while proteins associated with motility were predominantly modified in T. evansi. The establishment of a database of multiple PTMs in the two parasites provides us with insight into the biological mechanisms that underpin life processes in trypanosomes with huge different life cycles.

2020-06-30 | PXD016425 | Pride

Whole genome tomato transcriptional response to Tetranychus evansi herbivory.

Project description:The red spider mite, Tetranychus evansi, is a oligophagous specialist mite pest of Solanaceae plants. Here, we described tomato transcriptional responses to T. evansi feeding and compared them to responses to tomato-adapted and -non-adapted strains of generalist herbivorous spider mite Tetranychus urticae. We used microarray to assess global gene expression in Solanum lycopersicum cv. Heinz 1706 upon T. evansi attack.

2023-07-10 | GSE227740 | GEO

Stefania evansi

Project description:Stefania evansi

| PRJNA1169567 | ENA