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Differentially expressed genes between ACh-stimulated KYSE30 cells and the respective control cells

ABSTRACT: Differentially expressed genes between ACh-stimulated KYSE30 cells and the respective control cells

PROVIDER: PRJNA836279 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19139634_1.fastq.gz	Fastqsanger.gz
	SRR19139634_2.fastq.gz	Fastqsanger.gz
	SRR19139635_1.fastq.gz	Fastqsanger.gz
	SRR19139635_2.fastq.gz	Fastqsanger.gz
	SRR19139636_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Differentially expressed genes between ACh-stimulated KYSE30 cells and the respective control cells

Project description:Purpose:RNA sequencing explored the possible altered gene transcriptome and signal pathways between ACh-stimulated KYSE30 cells and the respective control cells. Methods:RNA sequencing explored the ACh-stimulated KYSE30 cells and the respective control cells.

2022-05-11 | GSE202482 | GEO

Transcriptomes Analysis of LHX2-knockdown in KYSE30 and KYSE510 cells

Project description:Purpose: To fully realize the potential molecular mechanism that LHX2 promotes ESCC progression Methods: Total RNA of LHX2-knockdown KYSE30/KYSE510 and control cells was extracted with TRIzol Reagent. RNA libraries were constructed using an Illumina TruSeq RNA Sample Preparation kit according to the manufacturer’s protocol. A total of 150 base paired-end reads were sequenced using the Novaseq 6000 S4 platform. Results: We identified 26008 transcripts in KYSE30 control and KYSE30 LHX2-knockdown cells ,and 25561 transcripts in KYSE510 control and KYSE510 LHX2-knockdown cells. Conclusions: Our study represents the analysis of LHX2-knockdown ESCC cells, generated by RNA-seq.

2023-04-07 | GSE209942 | GEO

RNA sequencing analysis of OTUD6B-knockdown KYSE30 and KYSE450 cells

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are using RNA sequencing analysis to test the effects of OTUD6B-knockdown on the mRNA of KYSE30 and KYSE450 cells. Methods: KYSE30 and KYSE450 cells were transfected with negative control or siOTUD6B for 48 hours in 1640 medium plus 10% serum. Three independent replicates were plated, transfected in parallel for each negative control and siOTUD6B. Results: Log-fold changes of mRNAs between negative control and siOTUD6B group were selected with a significance threshod of p<0.05. Conclusions: Our study determined the mRNA changes of OTUD6B-knockdown KYSE30 and KYSE450 cells.

2023-04-01 | GSE209521 | GEO

Effect of Fusobacterium nucleatum (Fn) infection or Fn-Dps treatment on gene expression of esophageal carcinoma Kyse150 and Kyse30 cells

Project description:Kyse150 and Kyse30 cells were infected by Fn (the MOI of 1:10) or treated with Fn-Dps (1 μM) for 48 h

2023-06-01 | GSE222917 | GEO

Expression analysis of gene expression chang in Homo sapiens Esophageal squamous cell carcinoma cells KYSE30 after knock down MTA2 gene

Project description:Investigation of whole genome gene expression level changes in Homo sapiens Esophageal squamous cell carcinoma cells KYSE30 after knock down of MTA2 gene expression

2021-03-10 | GSE112495 | GEO

Next generation sequencing facilitates transcriptome analyses of mock and RHCG transfected esophageal squamous cell carcinoma cell line KYSE30 cells

Project description:We obtained transcriptome profiling (RNA-seq) of human esophageal squamous cell carcinoma cell line KYSE30 cells stabley transfected with pIRES2-EGFP vector or human RHCG-expression vector by using next generation sequencing.

2019-09-26 | GSE87664 | GEO

Global expression analysis of miR-32/455/181a/181b overexpression or PI3K knockdown in KYSE30 cells

Project description:Analysis of the effect of the miRNA set (miR-32/455/181a/181b) on global gene expression. The hypothesis tested was that overexpression of these miRNAs would produce changes in gene expression similar to changes caused by PI3K knockdown. Data provided insight into genes that are disrupted with either individual miRNA and siRNA or the combination of miRNAs or siRNAs. Total RNA was isolated from KYSE30 cells transfected with either miRNA or siRNA for global expression analysis.

2012-12-29 | E-GEOD-43195 | biostudies-arrayexpress

RNA sequencing analysis of OTUD6B-knockdown KYSE30 and KYSE450 cells

Project description:RNA sequencing analysis of OTUD6B-knockdown KYSE30 and KYSE450 cells

| PRJNA861139 | ENA

Transcriptomes Analysis of LHX2-knockdown in KYSE30 and KYSE510 cells

Project description:Transcriptomes Analysis of LHX2-knockdown in KYSE30 and KYSE510 cells

| PRJNA863111 | ENA

Murine islets identified by TMT-labeled phosphoproteome after short time stimulation with neurotransmitters ACh and NE

Project description:Norepinephrine (NE) and acetylcholine (ACh) are important neurotransmitters of sympathetic and parasympathetic nerves, which play antagonistic roles in regulating different islet hormones secretion. Phosphorylation is reported as a critical post-translational modification participates in neural regulation on various physiological activities in islet. However, the molecular mechanisms of these two transmitters in islet are unknown and whether the neuronal regulation is abnormal in diabetes mellitus also unclear. Quantitative phosphoproteome analysis were performed on murine islet after short time stimulation with neurotransmitters NE and ACh to reveal the key moleculars and phosphorylated proteins in islet which participate in the innervation of nerve signaling.

2022-08-12 | PXD031491 | Pride

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