Dataset Information

Fetal growth restriction through parental and fetal immune gene variation and intercellular communications cascade

ABSTRACT: Fetal growth restriction through parental and fetal immune gene variation and intercellular communications cascade

PROVIDER: PRJNA837955 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	SRR19207400_1.fastq.gz	Fastqsanger.gz
	SRR19207400_2.fastq.gz	Fastqsanger.gz
	SRR19207500_1.fastq.gz	Fastqsanger.gz
	SRR19207500_2.fastq.gz	Fastqsanger.gz
	SRR19207600_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 5809

Similar Datasets

Project description:The dynamic protein distribution within and across the plasma membrane is pivotal in regulating cell communications. Yet, methods facilitating rapid labeling, applicable across diverse cell types, and amenable to multiplexed live imaging are scarce. We discovered that live mammalian cell surface proteins can be labeled using N-hydroxysuccinimide (NHS)-ester-based amine crosslinking of fluorescent dyes. Using mouse DC2.4 dendritic cells, human Jurkat T cells, and mouse primary lymphoma T cells as model systems, we revealed the membrane topology and membrane-associated cell communications previously challenging to capture by fluorescence microscopy techniques. In DC2.4 cells, we observed transient membrane protein accumulation at cell-cell contacts and their bidirectional migration patterns guided by membrane fibers. We further visualized the involvement of membrane fibers in the transfer of membrane-derived particles and ovalbumin, as well as caveolin-dependent endocytosis and subsequent transport of membrane labels in late endosomes and lysosomes. In addition, we demonstrate caveolin-1 phosphorylation-dependent insulin receptor endocytosis in HEK 293T cells. Volumetric structured illumination microscopy revealed changes in the membrane topology of Jurkat T cells in response to the activating surface, generation of membrane-derived microvesicles, and phagocytosis of CD3/CD28 Dynabeads on the glass surface. Finally, we demonstrate that the membrane label remains stable in the in vivo environment and evidence of intercellular membrane protein transfer from splenocytes from the donor T cell lymphoma SNF5 mouse to the healthy splenocytes in the C57/BL6 mouse 24 and 48-h after cell transfer. The ultrahigh membrane labeling density, coupled with high multiplexity and rapid labeling, enables innovative fluorescence microscopy applications to dissect membrane and membrane-derived structures in intercellular and intracellular communication both in vitro and in vivo.

Dataset Information

Fetal growth restriction through parental and fetal immune gene variation and intercellular communications cascade

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets