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CF (1565) vs CFTR-corrected (1564) iPSC-airway at ALI

ABSTRACT: CF (1565) vs CFTR-corrected (1564) iPSC-airway at ALI

PROVIDER: PRJNA838008 | ENA |

REPOSITORIES: ENA

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CF (1565) vs CFTR-corrected (1564) iPSC_airway at ALI

Project description:Platform: iPSC-derived airway plated on 2D-air liquid interface through basal cell intermediate. Purpose of experiment: To examine the role of mutant CFTR on IPSC-derived airway epithelium (ie. immune dysregulation; dysregulated calcium channel signaling etc). Description of samples:Typical phenotype CF F508del (1565) and syngeneic CFTR-corrected (1564) iPSCs- derived airway epithelium at D90 (D15 CD47hi/CD26lo; replated in 3DMG cultured in 210DCIY x 2 weeks, single-cell passaged and cultured in PNExPlus+dual smad media in 3D, s/p 2 NGFR sorts, cultured at air-liquid interface at 2D)

2023-03-02 | GSE203006 | GEO

CF (1565) vs CFTR-corrected (1564) iPSC_airway at ALI

Project description:CF (1565) vs CFTR-corrected (1564) iPSC_airway at ALI

| PRJNA838010 | ENA

CF (1565) vs CFTR-corrected (1564) iPSC-airway at ALI

Project description:Purpose of experiment :To 1) evaluate for the presence of pulmonary ionocytes, 2) investigate the role of the CFTR in airway epithelium. Description of samples: 1564 (CF-repaired), 1565 (CF DF508homozygous) iPSC-airway epithelium through basal cell intermediate. iPSC-D15CD47/CD26sort- NGFR sort x 2

2023-03-02 | GSE203008 | GEO

CF vs CFTR-corrected hiPSC-airway at ALI

Project description:CF vs CFTR-corrected hiPSC-airway at ALI

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CF (1567) vs CFTR-corrected (1566) iPSC-airway at ALI

Project description:CF (1567) vs CFTR-corrected (1566) iPSC-airway at ALI

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CF (1567) vs CFTR-corrected (1566) iPSC-airway at ALI

Project description:To interrogate the intrinsic effect of CFTR on airway epithelium; to interrogate the presence of FOXi1+ASLC3+ ionocyte population. Description of samples:1567 (F508del CF patient of rapid progressive phenotye) and 1566 (isogenic CFTR-repaired pair). iPSC-derived airway epithelium, s/p D15CD47hi/CD26lo sort, 210DCYI media x

2023-03-02 | GSE203007 | GEO

CF vs CFTR-corrected hiPSC-airway at ALI

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-03-02 | GSE203009 | GEO

Coemansia reversa NRRL 1564 isolate:NRRL 1564

Project description:Coemansia reversa NRRL 1564 transcriptome

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CFTR mutation abrogates Prevotella regulation of epithelial cell defense against Staphylococcus aureus

Project description:Background: The oral anaerobe Prevotella melaninogenica is enriched in the lungs of people with cystic fibrosis (pwCF), yet its functional impact on respiratory tract homeostasis remains incompletely understood. Prior studies identified immune modulatory effects following lung exposure to Prevotella, but the relevance of these findings for CF infections is unknown. Methods: The impact of P. melaninogenica on infection with the CF pathogen Staphylococcus aureus was evaluated using a mouse lung infection model and human respiratory tract cystic fibrosis transmembrane conductance regulator (CFTR) mutant and isogenic wild-type (WT)-corrected CFBE41o- epithelial cells. RNA-sequencing was performed to compare Prevotella-induced signaling programs in WT-corrected versus CFTR mutant cells. Results: P. melaninogenica significantly reduced S. aureus lung infection, which was associated with elevated S. aureus killing by lung neutrophils and impaired S. aureus adherence to epithelial cells. Live or heat-killed Prevotella were sufficient to mediate these effects, which were dependent on the toll-like receptor TLR2. Prevotella impairment of S. aureus adherence also required CFTR function, as this effect was lost in CFTR mutant cells but restored by CFTR modulator therapy. RNA-sequencing identified several antibacterial defense pathways selectively upregulated by Prevotella in WT corrected epithelial cells, correlating with higher IL-8 and IL-6 cytokine production. Conclusions: P. melaninogenica enhanced neutrophil and epithelial defense against S. aureus, but these benefits were lost with CFTR dysfunction. CFTR modulator therapy rescued Prevotella responsiveness in respiratory epithelial cells, highlighting the potential for synergistic effects of host-microbiome interactions and CFTR targeted therapies.

2025-12-09 | GSE305398 | GEO

Coemansia guatemalensis strain:NRRL 1565 | isolate:NRRL 1565

Project description:Coemansia guatemalense NRRL 1565 Resequencing genome sequencing

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