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Accessible chromatin differences in FCRL5 positive and negative influenza-specific memory B cells

ABSTRACT: Accessible chromatin differences in FCRL5 positive and negative influenza-specific memory B cells

PROVIDER: PRJNA838598 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19225807_1.fastq.gz	Fastqsanger.gz
	SRR19225807_2.fastq.gz	Fastqsanger.gz
	SRR19225808_1.fastq.gz	Fastqsanger.gz
	SRR19225808_2.fastq.gz	Fastqsanger.gz
	SRR19225809_1.fastq.gz	Fastqsanger.gz

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Accessible chromatin differences in FCRL5 positive and negative influenza-specific memory B cells

Project description:We examined the accessible chromatin differences between circulating B cell subsets post-vaccination. Using FCRL5 as a marker of Tbet expressing B cells, we sorted FCRL5+ (Tbet+) and FCRL5- (Tbet-) memory B cells, and antibody secreting plasmablasts at day 7 and day 14 post-vaccination. Additionally, we used H1 B cell tetramers to track cells responding to the influenza vaccine in a subset of samples. These data provide insights into the accessible chromatin landscape of distinct B cell subsets that respond to influenza vaccination.

2023-03-01 | GSE203112 | GEO

Transcriptome differences in FCRL5 positive and negative Influenza-specific memory B cells

Project description:We examined the transcriptional differences between circulating B cell subsets post-vaccination. Using FCRL5 as a marker of Tbet expressing B cells, we sorted FCRL5+ (Tbet+) and FCRL5- (Tbet-) memory B cells, naïve B cells, and antibody secreting plasmablasts at day 7 and day 14 post-vaccination. Additionally, we used H1 B cell tetramers to track cells responding to the influenza vaccine. These data provide insights into the transcriptional programming of distinct B cell subsets that respond to influenza vaccination.

2023-03-01 | GSE163989 | GEO

Transcriptome differences in FCRL5 positive and negative Influenza-specific memory B cells

Project description:Transcriptome differences in FCRL5 positive and negative Influenza-specific memory B cells

| PRJNA688481 | ENA

The memory B cell response to influenza vaccination is impaired in older persons

Project description:Influenza imparts an age-related increase in mortality and morbidity. The most effective countermeasure is vaccination; however, vaccines offer modest protection in older adults. To investigate how ageing impacts the memory B cell response we tracked haemagglutinin specific B cells by indexed flow sorting and single cell RNA sequencing in twenty healthy adults administered the trivalent influenza vaccine. We found age-related skewing in the memory B cell compartment six weeks after vaccination, with younger adults developing haemagglutinin specific memory B cells with an FCRL5+ “atypical” phenotype, showing evidence of somatic hypermutation and positive selection, which happened to a lesser extent in older persons. We confirmed the germinal center ancestry of these FCRL5+ “atypical” memory B cells using scRNASeq from fine needle aspirates of influenza responding human lymph nodes and paired blood samples. Together, this study shows that the aged human germinal center reaction and memory B cell response following vaccination is defective.

2021-03-05 | GSE167823 | GEO

Expansion of an FCRL5+ B cell subset resembling atypical memory B cells in an animal model of malaria

Project description:Background: In human malaria, parasites of the genus Plasmodium elicit expansion of atypical memory B cells (atMBCs), which lack the classical markers CD21 and CD27. We have identified a putative population of analogous B cells in a murine model of infection with P. chabaudi, delineated by the marker FCRL5. We performed RNA-Seq on FCRL5+ and FCRL5- B cells sorted from infected mice, so as to characterize the transcriptional profile of these cells and permit comparison to atMBCs in humans. Results: FCRL5+ B cells were found to have distinct transcriptional profiles from FCRL5- B cells, with approximately 400 genes exhibiting significant differences between the two groups. Additionally, about 25% of these differentially expressed genes were also differentially expressed in human atMBCs versus classical MBCs, as previously described by Sullivan et al (PLoS Pathogens 2015). Conclusions: FCRL5+ class-switched B cells are a transcriptionally distinct subset arising in P. chabaudi infection, with transcriptional similarities to human atMBCs that develop in chronic malaria settings.

2019-04-16 | GSE85205 | GEO

Fc receptor-like 4 (FCLR4) and Fc receptor-like receptor 5 (FCRL5) define and control human atypical memory B cells [RNA-Seq]

Project description:We performed NGS on Ig heavy chain V sequences amplified from RNA obtained from human tonsillar B cell subsets. From these NGS data, we reconstructed ongoing B cell responses as lineage trees, providing crucial in vivo developmental context. Each memory subset typically maintained its lineage, denoting mechanisms enforcing their phenotypes. FCRL4-FCRL5+ cells were the most represented memory subset in lineage trees, indicating robust participation in ongoing responses. Affinity maturation of both FCRL4-FCRL5+ and FCRL4+FCRL5+ atypical memory cells was stalled in chronic streptococcus infection, conceivably due to negative feedback from IgG immune complexes. We propose that FCRL4 and FCRL5 function as sensors of immune complexes, thus controlling atypical memory B cell responses.

2020-08-04 | GSE145519 | GEO

Comparison of gene expression profiles of FCRL5+/CD21-/lo/CD27- and FCRL5-/CD21-/lo/CD27- peripheral B cells from healthy donors

Project description:To compare gene expression profile of FCRL5+ and FCRL5- tissue-like memory B cells (CD21-/lo/CD27-/CD19+), we have employed whole genome microarray expression profiling to identify differentially regulated genes in these two B cell subgroups. B cells freshly isolated from leukapheresis of healthy donors were used in the experiment.

2016-04-26 | GSE66470 | GEO

Fc receptor-like 4 (FCLR4) and Fc receptor-like receptor 5 (FCRL5) define and control human atypical memory B cells [array]

Project description:We performed gene array analysis of three memnory cell subsets sorted from three tonsil donors with the following patterns of surface expression: FCRL4-, FCRL5+ (designated F5), FCRL4 -, FCRL 5- (designated DN) , and FCRL4+, FCRL5+ (designated DP). The three memory subsets were clearly segregated by principal component analysis and cluster analysis as distinct, and hundreds of genes were found differentially expressed. between any two subsets

2020-08-04 | GSE144089 | GEO

Fully accessible fitness landscape of oncogene negative lung adenocarcinoma

Project description:Fully accessible fitness landscape of oncogene negative lung adenocarcinoma

| PRJNA927274 | ENA

FCRL5<sup>+</sup> Memory B Cells Exhibit Robust Recall Responses.

Project description:FCRL5+ atypical memory B cells (atMBCs) expand in many chronic human infections, including recurrent malaria, but studies have drawn opposing conclusions about their function. Here, in mice infected with Plasmodium chabaudi, we demonstrate expansion of an antigen-specific FCRL5+ population that is distinct from previously described FCRL5+ innate-like murine subsets. Comparative analyses reveal overlapping phenotypic and transcriptomic signatures between FCRL5+ B cells from Plasmodium-infected mice and atMBCs from Plasmodium-exposed humans. In infected mice, FCRL5 is expressed on the majority of antigen-specific germinal-center-derived memory B cells (MBCs). Upon challenge, FCRL5+ MBCs rapidly give rise to antibody-producing cells expressing additional atypical markers, demonstrating functionality in vivo. Moreover, atypical markers are expressed on antigen-specific MBCs generated by immunization in both mice and humans, indicating that the atypical phenotype is not restricted to chronic settings. This study resolves conflicting interpretations of atMBC function and suggests FCRL5+ B cells as an attractive target for vaccine strategies.

| S-EPMC6530801 | biostudies-literature

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