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The association and difference of normal THP-1 cell and H37Rv infected THP-1 cell.

ABSTRACT: The association and difference of normal THP-1 cell and H37Rv infected THP-1 cell.

PROVIDER: PRJNA839277 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19264358_1.fastq.gz	Fastqsanger.gz
	SRR19264358_2.fastq.gz	Fastqsanger.gz
	SRR19264359_1.fastq.gz	Fastqsanger.gz
	SRR19264359_2.fastq.gz	Fastqsanger.gz
	SRR19264360_1.fastq.gz	Fastqsanger.gz

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The association and difference of normal THP-1 cell and H37Rv infected THP-1 cell.

Project description:①Background:Tuberculosis is mainly a respiratory tract infection caused by mycobacterium tuberculosis and one of the leading causes of death worldwide. According to the Global Tuberculosis Report in 2021, About a quarter of the world's population is infected with Mycobacterium tuberculosis and China is the second highest burden of TB. Although TB diagnosis and prevention techniques have become more mature, the number of TB cases is still increasing, mainly due to: the prevalence of drug-resistant tuberculosis bacteria, tuberculosis and HIV co-infection, long incubation time of mycobacterium tuberculosis difficult to early diagnosis and so on. Therefore, it is of great significance to study the pathogenesis of mycobacterium tuberculosis infection.②Method: THP-1 cells were treated with 50ng/ml PMA for 24 hours, so that THP-1 cell can be induced into macrophages. After that THP-1 macrophages were infected with mycobacterium tuberculosis H37Rv(MOI=1), which were collected and applied to RNA-sequencing. The constructed sequencing library was sequenced using an Illumina Novaseq 6000 system.

2022-08-31 | GSE203261 | GEO

THP-1 cells infected with H37Rv

Project description:Cells were seeded in 24-well plates with phorbol myristate (PMA, Sigma Aldrich, USA) at a concentration of 100 ng/ml. Adherent THP-1 cells were infected with H37Rv at an MOI of 1 for 4 hours, and then washed three times with PBS to remove bacteria. Experiments involving live H37Rv were performed in a P2+ facility.

2025-05-17 | GSE267737 | GEO

THP-1 infection H37Rv sequencing results

Project description:THP-1 infection H37Rv sequencing results

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THP-1 cells infected with H37Rv

Project description:THP-1 cells infected with H37Rv

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Temporal response of Mycobacterium tuberculosis H37Rv to vitamin C exposure in THP-1 macrophages.

Project description:Transcriptional profiling of Mtb H37Rv infected into THP-1 macrophage cell line and treated with 100 µM vitamin C (vit C) for 96 hours and 144 hours, compared to gene expression profile of untreated bacteria post-infection.

2021-10-01 | GSE179376 | GEO

Human mRNA profile of Mycobacterium tuberculosis H37Rv infected and hsa-miR-643 mimic transfected THP-1 differentiated macrophages cells.

Project description:The analysis of differentially regulated mRNAs candidates in THP-1 cells infected with H37Rv was conducted, comparing them to both uninfected THP-1 cells and transfected reference samples. THP-1 cells are monocytes that differentiate into macrophages after being treated with PMA for 48 hours. Total RNA was isolated from the infected and transfected THP-1 cells at 24 hours post-infection.

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Identification of genes differentially regulated upon infection of Thp-1 cells with H37Rv

Project description:Perturbation of host physiology by intracellular Mycobacterium tuberculosis (H37Rv) can be reflected by changes in gene expression pattern of host genes. Total RNA was isolated from PMA differentiated uninfected cells or cells infected with H37Rv for 16, 48 or 90 hours and gene expression profile was obtained. There are in all 8 samples, two replcates of each 4. Two samples, namely 1D,1E (replicates of one) are control (PMA differentiated Thp-1 cells Uninfected controls). Cells were infected with H37Rv at an MOI of 1:10 and samples were collected at 16 hours, 48 hours and 90 hours post-infection.

2010-05-27 | E-GEOD-19052 | biostudies-arrayexpress

TaqMan Human miRNA array profile of Mycobacterium tuberculosis H37Rv infected THP-1 differentiated macrophages cells.

Project description:Differentially regulated miRNA candidates in H37Rv infected THP-1 cells were analysed with respect to uninfected THP-1 reference samples. THP-1 cells are monocytes differentiated to macrophages after treatment with PMA for 48 hrs. Total RNA was isolated from infected THP-1 cells after 24 hrs of infection, cDNA was synthesized for TLDA real time PCR reaction using TaqMan MicroRNA Reverse Transcription kit and Megaplex Human Pool A and Pool B stem loop RT primers (version 3.0) as per manufacturer’s protocol. Further real time reaction was performed on QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems) by using cDNA (without pre-amplification) on TLDA card A and card B (version 3.0).

2017-04-20 | GSE94007 | GEO

THP-1 infection H37Rv sequencing results

Project description:High-throughput sequencing was conducted to identify the effector molecules regulated by H37rv.

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Real-time quantitative PCR analysis of PMA differentiated THP-1 infected with H37Rv or M. marinum, or heat killed H37Ra (HKMT)

Project description:THP-1 cells were differentiated into macrophages with 0,1 µM PMA overnight then infected for 2 days with either H37Rv, M. marinum or heat killed H37Ra. Then RNA was collected in order to quantify the inflammatory response transcription as compared with the relative uninfected controls.

2021-07-20 | GSE165327 | GEO

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