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Analysis of cellular transcripts targeted by ZAPL or ZAPS in HEK293T cells

ABSTRACT: Analysis of cellular transcripts targeted by ZAPL or ZAPS in HEK293T cells

PROVIDER: PRJNA841224 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19347613_1.fastq.gz	Fastqsanger.gz
	SRR19347613_2.fastq.gz	Fastqsanger.gz
	SRR19347614_1.fastq.gz	Fastqsanger.gz
	SRR19347614_2.fastq.gz	Fastqsanger.gz
	SRR19347615_1.fastq.gz	Fastqsanger.gz

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Analysis of cellular transcripts targeted by ZAPL or ZAPS in HEK293T cells

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Gene expression analysis of HEK293T cells after CHMP5 gene modulation

Project description:Microarray-based gene expression analysis identified genes differentially expressed in HEK293T cells after CHMP5 gene modulation. In this study, HEK293T cells, silenced for CHMP5 using sh-CHMP5 was compared to genome wide expression changes when compared to cellular transfections with a sh-luciferase control by Significance Analysis of Microarray data. Further, overexpression of CHMP5 using the vector pCMV_CHMP5 was compared to cellular transfections with an empty vector pCMV in the cell line HEK293T

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Genome-wide analysis of miRNA-targeted cellular NMD substrates in HeLa cell

Project description:Analysis of miRNA-targeted cellular NMD substrates in HeLa cell. The hypothesis tested in the present study was that endogenous NMD substrates containing long 3' untranslated region may targeted for miRNA. Results provide important information expanding the roles of miRISC in the posttranscriptional regulation of gene expression: a new cross-talk between miRNA-mediated gene silencing and NMD. ABSTRACT: Imperfect base-pairing between microRNA (miRNA) and the 3â-untranslated region (3âUTR) of target mRNA triggers translational repression of the target mRNA. Here, we provide evidence that human Argonaute 2 (Ago2) targets cap-binding protein (CBP)80/20- and exon junction complex (EJC)-bound mRNAs and inhibits nonsense-mediated mRNA decay (NMD), which is tightly restricted to CBP80/20-bound mRNAs. Furthermore, microarray analyses reveal that a subset of cellular transcripts, which are expected to be targeted for NMD, is stabilized by miRNA-mediated gene silencing. The regulation of NMD by miRNAs will shed light on a new post-transcriptional regulation mechanism of gene expression in mammalian cells Total RNA obtained from HeLa cells with downregulation of Ago2 or Ago2/UPF1 by siRNA. The up- or down-regulated transcripts were compared to control siRNA treated HeLa cell RNA extract. Significant transcripts were confirmed by replication.

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Transcriptome analysis of targeted RNA-knockdown using CRISPR-Csm in HEK293T cells

Project description:Transcriptome analysis of targeted RNA-knockdown using CRISPR-Csm in HEK293T cells

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Transcriptome analysis of targeted RNA-knockdown using CRISPR-Csm in HEK293T cells

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Limited Evidence for Protein Products of Noncoding Transcripts in the HEK293T Cellular Cytosol.

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| S-EPMC9396073 | biostudies-literature

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