Project description:To confirm the transcriptional mechanism of xylose gene activation in Rhodotorula toruloides is principally determined by acetate and not other factors, like nitrogen content. RNA-Seq was conducted to understand xylose pathway metabolism. Ultimately, we used new-found understanding in RNA-Seq to engineer R. toruloides better.
Project description:This RNA-seq study examines transcriptional responses of a cyanobacterium–yeast co-culture system comprising Synechococcus elongatus PCC 7942 and Rhodotorula toruloides NBRC 0880 (Rhodosporidium toruloides IFO0880) under multiple conditions. Experiments included planktonic co-cultures in 50 mL tubes subjected to chemical stressors (oxidative stress, photosynthesis inhibition), temperature stress, nutrient limitation (nitrogen, phosphorus), pH extremes, and heterotrophic conditions, as well as physically separated co-cultures in a 600 mL membrane bioreactor. Axenic controls for Synechococcus elongatus PCC 7942 were included for comparison. Differential gene expression analysis provides insight into interspecies interactions and individual organism responses across co-culture configurations.
