Project description:The black soldier fly, Hermetia illucens, is a promising source for sustainable production of proteins, lipids and bioactive substances. Feeding on a variety of decomposing organic substrates which are typically inhabited by a range of different microorganisms like bacteria and fungi, H. illucens larvae is expected to be able to strongly produce antimicrobial peptides (AMPs) and other substances possessing antibiotic activity. It has been shown that H. illucens larvae extract exhibits antimicrobial activity against some microorganisms, such as Escherichia coli and Staphylococcus aureus, and antimicrobial effect on bacterial biofilm. Numbers of AMPs produced by H. illucens larvae have also been identified so far. Moreover, H. illucens larvae extract was recently observed with significant induction of cell envelop stress response (CESR) in B. subtilis, indicative of potential production of antimicrobial compounds. B. subtilis as a well-established Gram‑positive model strain is widely used in scientific research and an arguably robust platform to investigate antimicrobial compounds at the respect of, such as the mode of reaction of and the stress response elicited by corresponding compounds. In this study, we aimed to characterize the stress responses in B. subtilis induced by H. illucens larvae extract by employing transcriptomic study via RNA sequencing technique. With transcriptional profiling, we are expecting to be able to give some hints of potential antimicrobial compounds produced by H. illucens larvae by referring to the transcriptomic researches on well-studied antimicrobial substances.
Project description:The larvae of black soldier fly (BSF), Hermetia illucens L., are of economic importance due to their potential as livestock feed. However, the knowledge on their immune response to infection and wounding remains limited. In this study, we uncover the transcriptomic response of BSF larvae to wounding as well as infection with Gram-negative pathogen, Pseudomonas protegens Pf-5 at multiple timepoints. We observed that initially the BSF larvae generated a common immune response to both wounding and infection. However, the immune response is only upregulated in infected larvae over time, while the gene expression in response to wounding reduced over time. Genes encoding for signalling molecules such as PGRP-SA, Relish as well as genes encoding antimicrobial peptides like cecropin, defensin and attacin contributed primarily to BSF larvae's immune responses to both wounding as well as infection.
2025-06-01 | GSE263745 | GEO
Project description:the intestinal microbiota of black soldier fly larvae (Hermetia illucens L.)
Project description:The present study was conducted to investigate the effect of graded levels of black soldier fly larvae (BSFL) (Hermetia illucens) meal and BSFL paste in extruded diets for Atlantic salmon (Salmo salar). A total of 1260 Atlantic salmon with 34 g of mean initial weight were randomly distributed into 21 fiberglass tanks and fed (n=3) with seven extruded isolipidic and isonitrogenous diets for seven weeks. The experimental diets consisted of a positive control diet based on fishmeal, soy protein concentrate, corn gluten, faba bean and fish oil (Control_1); three diets with increased levels of full lipid BSFL meal, substituting 6.25% (6.25_IM), 12.5% (12.5_IM) and 25% (25_IM) of the protein content of Control_1; two diets with increased levels of full lipid BSFL paste, substituting 3.7% (3.7_IP) and 6.7% (6.7_IP); and of protein from Control_1 and a negative a control with 0.84 % of formic acid (Control_2). We investigate the effect of diets on growth performance, mmune response and health.