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A grainyhead transcription factor Tfcp2l1 controls the timing for lung alveolar regeneration (scRNA-Seq)

ABSTRACT: A grainyhead transcription factor Tfcp2l1 controls the timing for lung alveolar regeneration (scRNA-Seq)

PROVIDER: PRJNA842346 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19395594_1.fastq.gz	Fastqsanger.gz
	SRR19395594_2.fastq.gz	Fastqsanger.gz
	SRR19395595_1.fastq.gz	Fastqsanger.gz
	SRR19395595_2.fastq.gz	Fastqsanger.gz

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A grainyhead transcription factor Tfcp2l1 controls the timing for lung alveolar regeneration

Project description:A grainyhead transcription factor Tfcp2l1 controls the timing for lung alveolar regeneration

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A grainyhead transcription factor Tfcp2l1 controls the timing for lung alveolar regeneration (scRNA-Seq)

Project description:Alveolar epithelial type 2 (AT2) cells are facultative progenitor cells that drive adult alveolar regeneration after acute lung injury. Using transcriptomic analyses from in vivo mouse injury models, we define the role of Tfcp2l1 in regulating AT2 cell behavior during lung regeneration.

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A grainyhead transcription factor Tfcp2l1 controls the timing for lung alveolar regeneration (RNA-Seq)

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A grainyhead transcription factor Tfcp2l1 controls the timing for lung alveolar regeneration (RNA-Seq)

Project description:A grainyhead transcription factor Tfcp2l1 controls the timing for lung alveolar regeneration (RNA-Seq)

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A grainyhead transcription factor Tfcp2l1 controls the timing for lung alveolar regeneration

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Temporal and spatial staging of lung alveolar regeneration is determined by the grainyhead transcription factor Tfcp2l1.

Project description:Alveolar epithelial type 2 (AT2) cells harbor the facultative progenitor capacity in the lung alveolus to drive regeneration after lung injury. Using single-cell transcriptomics, software-guided segmentation of tissue damage, and in vivo mouse lineage tracing, we identified the grainyhead transcription factor cellular promoter 2-like 1 (Tfcp2l1) as a regulator of this regenerative process. Tfcp2l1 loss in adult AT2 cells inhibits self-renewal and enhances AT2-AT1 differentiation during tissue regeneration. Conversely, Tfcp2l1 blunts the proliferative response to inflammatory signaling during the early acute injury phase. Tfcp2l1 temporally regulates AT2 self-renewal and differentiation in alveolar regions undergoing active regeneration. Single-cell transcriptomics and lineage tracing reveal that Tfcp2l1 regulates cell fate dynamics across the AT2-AT1 differentiation and restricts the inflammatory program in murine AT2 cells. Organoid modeling shows that Tfcp2l1 regulation of interleukin-1 (IL-1) receptor expression controlled these cell fate dynamics. These findings highlight the critical role Tfcp2l1 plays in balancing epithelial cell self-renewal and differentiation during alveolar regeneration.

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