Project description:We performed Proteomics on the isolated stratum corneum from adult frog skin, hindlimb toes without claws, and hindlimb toes which bear cornified claws. We aimed to identify yet undescribed genes relevant for cornification which are arranged in a cluster which we named Amphibian Epidermal Differentiation Cluster (AEDC). We needed to use the protease GludC instead of Trypsin in order to obtain detectable peptides.
2026-08-13 | PXD073929 | Pride
Project description:amphibian skin microbiome.
| PRJNA916214 | ENA
Project description:Phylosymbiosis of amphibian microbiomes
| PRJNA872146 | ENA
Project description:Global survey of amphibian microbiomes
Project description:Photoaging-induced deterioration of skin structural integrity and barrier function represents a critical challenge in dermatological health. While small extracellular vesicles (sEVs) hold therapeutic potential, their source diversity remains underexplored. Here, we report a novel sEV, designated OA-UVR-sEV, isolated from ultraviolet irradiation (UVR)-stimulated skin secretions of the amphibian Odorrana andersonii. Characterization confirmed its typical vesicular ultrastructure and excellent biocompatibility. Integrated multi-omics analysis revealed enrichment of functional miRNAs, proteins, and small-molecule metabolites with anti-photoaging potential. In vitro, OA-UVR-sEV substantially reduced reactive oxygen species accumulation, UVR-associated DNA damage marker expression, and pro-inflammatory cytokines in human immortalized keratinocytes and mouse dermal fibroblasts. For in vivo delivery, we developed a hyaluronic acid microneedle system, which enabled OA-UVR-sEV to attenuate wrinkle formation, stimulate dermal collagen synthesis, improve skin hydration, and reduce transepidermal water loss in photoaged mice. Analyses in dermal fibroblasts showed that these protective effects were associated with reduced activation of the MAPK and NF-κB signaling pathways. Collectively, our results establish OA-UVR-sEV as an effective anti-photoaging agent and highlight amphibian-derived sEVs as a promising new avenue for combating skin aging.
