Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiling of NUAK kinase overexpression in Drosophila larval muscle development

ABSTRACT: Gene expression profiling of NUAK kinase overexpression in Drosophila larval muscle development

PROVIDER: PRJNA842684 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19416933_1.fastq.gz	Fastqsanger.gz
	SRR19416933_2.fastq.gz	Fastqsanger.gz
	SRR19416934_1.fastq.gz	Fastqsanger.gz
	SRR19416934_2.fastq.gz	Fastqsanger.gz
	SRR19416935_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 36

Similar Datasets

Gene expression profiling of NUAK kinase overexpression in Drosophila larval muscle development

Project description:Signal transduction pathways mediated by kinases control diverse biological outputs at the level of cells and tissues to regulate a diverse array of biological and developmental events. To gain insight into how muscle expression of the evolutionarily conserved NUAK kinase regulates the transcriptional landscape during Drosophila melanogaster development, we performed high-throughput sequencing of RNA from either whole larvae or dissected muscle fillets at the end of larval development. Raw data was generated using the Illumina HiSeq platform. After trimming and mapping to the Drosophila reference genome, differential gene expression and GO enrichment analysis were completed.

2022-05-30 | GSE204894 | GEO

RNA-seq analysis to profile TGF-beta/Activin signaling-dependent gene expression in Drosophila larval skeletal muscle

Project description:We performed RNA-seq analysis to identify genes whose expression is regulted or influenced by TGF-beta/Activin signaling in Drosophila larval skeletal muscle. We used two sample sets. One is composed of wild-type and smox (the sole R-smad of TGF-beta/Activin signaling in Drosophila). The other is composed of control and larvae of muscle-specific expression of activated Baboo (Type I receptor). By comparing differential expressions from these two sets, we were able to identify genes whose expression is regulated by TGF-beta/Activin pathway in the skeletal muscle of Drosophila larvae.

2020-12-08 | GSE162810 | GEO

Loss of Antp function in Drosophila flight muscle precursors : RNAi in larval wing disc-associated myoblasts

Project description:Muscle development and diversity require a large number of spatially and temporally regulated events controlled by transcription factors (TFs). Drosophila has long stood as a model organism to study myogenesis due to the highly conserved key TFs involved at all stages of muscle development, from specification to differentiation. While many studies focused on the diversification of Drosophila larval musculature, how distinct adult muscle types are generated is much less characterised. Here, we identify a novel essential regulator of Drosophila thoracic flight muscle development, the Hox TF Antennapedia (Antp). Correcting a long standing misconception that flight muscle development occurs without the input of Hox TFs, we show that Antp intervenes at several stages of adult flight muscle development, from the establishment of the progenitor pool in the embryo to myoblast differentiation in the early pupa. Furthermore, the precisely regulated clearance of Hox at the pupal stage in the developing indirect flight muscle fibers is required to allow for stretch-activated fibrillar muscle fate diversification that sets these flight muscles apart from all other adult tubular muscle types.

2024-10-23 | GSE268578 | GEO

Mutations in the Drosophila melanogaster gene pyruvate kinase interfere with larval development

Project description:Pyruvate kinase (Pyk) is a rate-limiting enzyme that catalyzes the final metabolic reaction in glycolysis. The importance of this enzyme, however, extends far beyond ATP production, as Pyk is also known to regulate tissue growth, cell proliferation, and development. Studies of this enzyme in Drosophila melanogaster, however, are complicated by the fact the fly genome encodes six Pyk paralogs and the function of these individual genes remain poorly defined. To address this issue, we used a phylogenetic approach to demonstrate that the gene Pyk encodes the enzyme most similar to the mammalian Pyk orthologs while the other five Drosophila Pyk have significantly diverged from the canonical enzyme. Consistent with this observation, metabolomic studies of two different Pyk mutant backgrounds revealed that larvae lacking Pyk exhibit a severe block in glycolysis, with a buildup of glycolytic intermediates upstream of pyruvate. However, our analysis also unexpectantly reveals that steady state pyruvate levels are unchanged in Pyk mutants, indicating that larval metabolism maintains pyruvate pool size despite severe metabolic limitations. Consistent with our metabolomics findings, a complementary RNAseq analysis revealed that genes involved in lipid metabolism and peptidase activity are elevated in Pyk mutants, again indicating that loss of this glycolytic enzyme induces compensatory changes in other aspects of metabolism. Overall, our study provides both insight towards understanding how Drosophila larval metabolism adapts to disruption of glycolytic metabolism and immediate clinical relevance considering that Pyk deficiency is the most common congenital enzymatic defect in humans.

2023-09-01 | GSE234299 | GEO

Mutations in the Drosophila melanogaster gene pyruvate kinase interfere with larval development

Project description:Mutations in the Drosophila melanogaster gene pyruvate kinase interfere with larval development

| PRJNA980708 | ENA

The Alk receptor tyrosine kinase regulates Sparkly, a novel activity regulating neuropeptide precursor in the Drosophila CNS

Project description:Numerous roles for the Alk receptor tyrosine kinase have been described in Drosophila, including functions in the CNS, however the molecular details are poorly understood. To gain mechanistic insight, we employed Targeted DamID (TaDa) transcriptional profiling to identify targets of Alk signaling in the larval CNS. TaDa was employed in larval CNS tissues, while genetically manipulating Alk signaling output. The resulting TaDa data were analysed together with larval CNS scRNA-seq datasets performed under similar conditions, identifying a role for Alk in the transcriptional regulation of neuroendocrine gene expression. Further integration with bulk/scRNA-seq and protein datasets from larval brains in which Alk signaling was manipulated, identified a previously uncharacterized Drosophila neuropeptide precursor encoded by CG4577 as an Alk signaling transcriptional target. CG4577, which we named Sparkly (Spar), is expressed in a subset of Alk-positive neuroendocrine cells in the developing larval CNS, including circadian clock neurons. In agreement with our TaDa analysis, overexpression of the Drosophila Alk ligand Jeb resulted in increased levels of Spar protein in the larval CNS. We show that Spar protein is expressed in circadian (Clock) neurons, and Spar mutants exhibit defects in sleep and circadian rhythm control. In summary, we report a novel activity regulating neuropeptide precursor gene that is regulated by Alk signaling in the Drosophila CNS.

2024-03-05 | GSE229518 | GEO

Gene expression profiling of NUAK kinase overexpression in <i>Drosophila</i> larval muscle development.

Project description:Signal transduction pathways mediated by kinases control diverse biological outputs at the level of cells and tissues to regulate a diverse array of biological and developmental events. To gain insight into how muscle expression of the evolutionarily conserved NUAK kinase regulates the transcriptional landscape during Drosophila melanogaster development, we performed high-throughput sequencing of RNA from either whole larvae or dissected muscle fillets at the end of larval development. Raw data was generated using the Illumina HiSeq 4000 platform. After trimming and mapping to the Drosophila reference genome, differential gene expression and GO enrichment analysis were completed. Raw data are deposited in the NCBI Gene Expression Ominbus (GEO) repository under GEO accession GSE204894.

| S-EPMC9340529 | biostudies-literature

Transcriptomic analysis of Drosophila larval and adult blood cells

Project description:Drosophila adult blood cells originate from larval peripheral hemocytes (derived from the embryonic waves of hematopoiesis) and from larval lymph gland hemocytes. To gain insight into the expression repertoire of Drosophila blood cells, we established the gene expression profiles the adult blood cells and their ascendants.

2021-09-28 | GSE174107 | GEO

RNA-sequencing analysis of Drosophila Hpo overexpression and knockdown in larval fat tissue

Project description:RNA-sequencing analysis of Drosophila Hpo overexpression and knockdown in larval fat tissue

| PRJNA833439 | ENA

Transcriptome analysis of Drosophila whole larval lymph glands

Project description:The balance between hematopoietic progenitors and differentiated hemocytes is finely tuned during development. In the larval hematopoietic organ of Drosophila, called the lymph gland, the receptor tyrosine kinase Pvr signals from differentiated cells to maintain a pool of undifferentiated progenitors. However, little is known about the processes that support Pvr function. The small GTPase Ral is involved in the regulation of several membrane trafficking events. Drosophila has a single Ral protein, Rala, which has been implicated in the development of various tissues. Here, we investigated the involvement of Rala in the larval fly hematopoietic system. We discovered that the loss of Rala activity phenocopies Pvr loss of function by promoting hemocyte progenitor differentiation. Moreover, using epistasis analysis, we found that the guanine exchange factor RalGPS lies upstream of Rala in this event, whereas the exocyst and Rab11 are acting downstream. Strikingly, the loss of Rala activity leads to a considerable accumulation of Pvr at the plasma membrane, hence suggesting a trafficking defect and reduced Pvr function. Consistent with this hypothesis, Rala loss of function phenotype in the lymph gland is fully suppressed by constitutive STAT activity, which normally mediates Pvr function in the lymph gland. Together, our findings unravel a novel RalGPS-Rala-exocyst-Rab11 axis for the maintenance of lymph gland homeostasis through Pvr.

2020-05-25 | GSE148035 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data