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Recycling of H2A-H2B provides short-term memory of chromatin states [SCAR-Seq]

ABSTRACT: Recycling of H2A-H2B provides short-term memory of chromatin states [SCAR-Seq]

PROVIDER: PRJNA843044 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19430600.fastq.gz	Fastqsanger.gz
	SRR19430601.fastq.gz	Fastqsanger.gz
	SRR19430602.fastq.gz	Fastqsanger.gz
	SRR19430603.fastq.gz	Fastqsanger.gz
	SRR19430604.fastq.gz	Fastqsanger.gz

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Recycling of H2A-H2B provides short-term memory of chromatin states [SCAR-Seq]

Project description:Chromatin landscapes are disrupted during DNA replication and must be restored faithfully to maintain genome regulation and cell identity. The H3-H4 modification landscape is restored by parental histone recycling and post-replication modification of new histone H3-H4. How DNA replication impact on histone H2A-H2B is unknown. Here, we track H2A-H2B modifications and H2A.Z during DNA replication and across the cell cycle using quantitative genomics. We show that H2AK119ub, H2BK120ub, and H2A.Z are recycled quantitatively and accurately during DNA replication. H2A-H2B are recycled symmetrically to daughter strands largely independent of known H3-H4 recycling pathways. Post-replication, H2A-H2B modifications are rapidly restored, and the rapid wave of H2AK119ub supports accurate restoration of H3K27me3. This work reveals epigenetic transmission of H2A-H2B modification during DNA replication and identifies H3-H4 and H2A-H2B crosstalk in epigenome propagation. We propose that rapid short-term memory of recycled H2A-H2B modifications facilitates reestablishment of slow, long-term chromatin state memory.

2023-02-06 | GSE204984 | GEO

Recycling of Histones H2A-H2B provides short-term memory of chromatin states [SCAR-seq II]

Project description:Chromatin landscapes are disrupted during DNA replication and need to be restored faithfully to maintain appropriate gene expression, including post-translational modifications (PTMs) of newly deposited histones. Whether histones H2A-H2B are accurately recycled during DNA replication and the behaviour of their associated marks during and post replication is unknown. Here we comprehensively map key modifications on H2A-H2B including H2A.Z during DNA replication. We show that H2AK119ub, H2BK120ub, and H2A.Z are recycled quantitatively and accurately during DNA replication in a symmetrical manner. Recycling occurs independently from H3-H4 chaperone pathways apart from a minor role for Polymerase alpha. H2A-H2B modifications are restored rapidly post replication and are important for timely and accurate restoration of H3-H4 marks, such as H3K27me3. This work uncovers H3-H4 and H2A-H2B crosstalk in epigenome propagation across DNA replication and suggests a model where rapid short-term memory of recycled H2A-H2B marks facilitates reestablishment of slow, long-term memory chromatin states.

2023-02-06 | GSE222928 | GEO

Recycling of H2A-H2B provides short-term memory of chromatin states [ChOR-Seq]

2023-02-06 | GSE204981 | GEO

Recycling of H2A-H2B provides short-term memory of chromatin states [ChIP-Seq]

2023-02-06 | GSE204974 | GEO

Recycling of Histones H2A-H2B provides short-term memory of chromatin states [SCAR-seq II]

Project description:Recycling of Histones H2A-H2B provides short-term memory of chromatin states [SCAR-seq II]

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Recycling of H2A-H2B provides short-term memory of chromatin states

Project description:Recycling of H2A-H2B provides short-term memory of chromatin states

| PRJNA843043 | ENA

Recycling of H2A-H2B provides short-term memory of chromatin states [ChOR-Seq]

Project description:Recycling of H2A-H2B provides short-term memory of chromatin states [ChOR-Seq]

| PRJNA843045 | ENA

Recycling of H2A-H2B provides short-term memory of chromatin states [ChIP-Seq]

Project description:Recycling of H2A-H2B provides short-term memory of chromatin states [ChIP-Seq]

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Recycling of modified H2A-H2B provides short-term memory of chromatin states.

Project description:Chromatin landscapes are disrupted during DNA replication and must be restored faithfully to maintain genome regulation and cell identity. The histone H3-H4 modification landscape is restored by parental histone recycling and modification of new histones. How DNA replication impacts on histone H2A-H2B is currently unknown. Here, we measure H2A-H2B modifications and H2A.Z during DNA replication and across the cell cycle using quantitative genomics. We show that H2AK119ub1, H2BK120ub1, and H2A.Z are recycled accurately during DNA replication. Modified H2A-H2B are segregated symmetrically to daughter strands via POLA1 on the lagging strand, but independent of H3-H4 recycling. Post-replication, H2A-H2B modification and variant landscapes are quickly restored, and H2AK119ub1 guides accurate restoration of H3K27me3. This work reveals epigenetic transmission of parental H2A-H2B during DNA replication and identifies cross talk between H3-H4 and H2A-H2B modifications in epigenome propagation. We propose that rapid short-term memory of recycled H2A-H2B modifications facilitates restoration of stable H3-H4 chromatin states.

| S-EPMC9994263 | biostudies-literature

Molecular basis of RanGTP-activated release of Histones H2A-H2B from Importin-9

Project description:Imp9 is the primary importin for shuttling H2A-H2B from the cytoplasm to the nucleus. It employs an unusual mechanism where the binding of RanGTP is insufficient to release H2A-H2B. The resulting stable RanGTP•Imp9•H2A-H2B complex gains nucleosome assembly activity with H2A-H2B able to be deposited into an assembling nucleosome in vitro. Using hydrogen-deuterium exchange coupled with mass spectrometry (HDX), we show that Imp9 stabilizes H2A-H2B beyond the direct binding site, like other histone chaperones. HDX also shows that binding of RanGTP releases H2A-H2B contacts at Imp9 HEAT repeats 4-5, but not 18-19. DNA- and histone-binding surfaces of H2A-H2B are exposed in the ternary complex, facilitating nucleosome assembly. We also reveal that RanGTP has a weaker affinity for Imp9 when H2A-H2B is bound. Imp9 thus provides a connection between the nuclear import of H2A-H2B and its deposition into chromatin.

2023-06-02 | PXD037571 | Pride

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