Project description:The ACTB gene, traditionally considered a reference gene, exhibits transcriptional instability in colorectal cancer tissues. The aim of this study was to evaluate the transcriptomic profile of colorectal adenocarcinoma, with a specific focus on structural genes and their regulatory networks within the tumor microenvironment. We conducted high-throughput mRNA profiling (Affymetrix GeneChip Human Gene 2.0 ST) of colorectal tumor tissues and healthy colonic mucosa. In parallel, a pilot microRNA screening (Affymetrix GeneChip miRNA 4.0) was performed on a subset of samples to uncover potential tumor-specific rewiring of regulatory networks involving ACTB and tumor-suppressive miRNAs.