Erynnis
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA843525 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Sperm dimorphism, the production of two distinct sperm morphs by a single male, is a widespread but poorly understood reproductive phenomenon. In Lepidoptera, fertilizing eupyrene sperm coexist with anucleate apyrene sperm, which cannot fertilize eggs but are nevertheless required for successful reproduction. Despite the prevalence and presumed adaptive significance of sperm dimorphism, the molecular basis of this trait remains poorly understood. Here, we characterize the proteome of dimorphic sperm in the Cabbage White butterfly, Pieris rapae, an emerging model for sexual selection and postcopulatory interactions. Using high-resolution, label-free mass spectrometry, we identified more than 1,600 proteins, nearly doubling the number previously reported for other lepidopteran species. Differential abundance analyses revealed eupyrene sperm were enriched for proteins linked to ion transport and vacuolar acidification, while apyrene sperm were enriched for mitochondrial and respiratory functions. Unexpectedly, comparative homology analyses with two other Lepidoptera, Danaus plexippus and Manduca sexta, showed P. rapae shared more homologous sperm proteins with M. sexta than with the more closely related D. plexippus, highlighting complex evolutionary dynamics of sperm proteomes. Together, these findings expand our understanding of sperm function and diversity in Lepidoptera, highlight the distinct roles of eupyrene and apyrene sperm, and provide a foundation for future studies of sperm evolution, sexual selection, and reproductive protein function.
2026-03-23 | PXD070767 | Pride
Project description:Technology for crosslinking and immunoprecipitation followed by sequencing (CLIP-seq) has identified the transcriptomic targets of hundreds of RNA-binding proteins in cells. To improve the power of existing and future CLIP-seq datasets, we introduce Skipper, an end-to-end workflow that converts unprocessed reads into annotated binding sites using an improved statistical framework. Compared to existing methods, Skipper on average calls 3.1-4.2 times more transcriptomic binding sites and sometimes >10 times more sites, providing deeper insight into post-transcriptional gene regulation. Skipper also calls binding to annotated repetitive elements and identifies bound elements for 99% of enhanced CLIP experiments. We perform nine translation factor enhanced CLIPs and apply Skipper to learn determinants of translation factor occupancy including transcript region, sequence, and subcellular localization. Furthermore, we observe depletion of genetic variation in occupied sites and nominate transcripts subject to selective constraint because of translation factor occupancy. Skipper offers fast, easy, customizable analysis of CLIP-seq data.
2022-10-08 | GSE213867 | GEO
Project description:Genomic analysis reveals a new genus of Firetip skippers (Lepidoptera: Hesperiidae: Pyrrhopyginae)
| PRJNA889040 | ENA
Project description:MicroRNAs (miRNAs) are involved in post-transcriptional regulation of gene expression. Since several miRNAs are known to affect the stability or translation of developmental regulatory genes, the origin of novel miRNAs may have contributed to the evolution of developmental processes and morphology. Lepidoptera (butterflies and moths) is a species-rich clade with a well-established phylogeny and abundant genomic resources, thereby representing an ideal system in which to study miRNA evolution. We sequenced small RNA libraries from developmental stages of two divergent lepidopterans, Cameraria ohridella (Horse chestnut Leafminer) and Pararge aegeria (Speckled Wood butterfly), discovering 90 and 81 conserved miRNAs respectively, and many species-specific miRNA sequences. Mapping miRNAs onto the lepidopteran phylogeny reveals rapid miRNA turnover and an episode of miRNA fixation early in lepidopteran evolution, implying that miRNA acquisition accompanied the early radiation of the Lepidoptera. One lepidopteran-specific miRNA gene, miR-2768, is located within an intron of the homeobox gene invected, involved in insect segmental and wing patterning. We identified cubitus interruptus (ci) as a likely direct target of miR-2768, and validated this suppression using a luciferase assay system. We propose a model by which miR-2768 modulates expression of ci in the segmentation pathway and in patterning of lepidopteran wing primordia.
2014-11-26 | GSE63644 | GEO