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PolyA+ RNA-Seq profiling of Drosophila melanogaster late third instar larval ovary, testis and sex transformed gonads as a result of tra and dsx perturbation.

ABSTRACT: PolyA+ RNA-Seq profiling of Drosophila melanogaster late third instar larval ovary, testis and sex transformed gonads as a result of tra and dsx perturbation.

PROVIDER: PRJNA844981 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19534920.fastq.gz	Fastqsanger.gz
	SRR19534921.fastq.gz	Fastqsanger.gz
	SRR19534922.fastq.gz	Fastqsanger.gz
	SRR19534923.fastq.gz	Fastqsanger.gz
	SRR19534924.fastq.gz	Fastqsanger.gz

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PolyA+ RNA-Seq profiling of Drosophila melanogaster late third instar larval ovary, testis and sex transformed gonads as a result of tra and dsx perturbation.

Project description:We designed this experiment to investigate the transcriptional changes in gonads as a result of sex transformation. Here we performed transcriptional profiling of the ovary transformed into testis from the tra loss of function (XX_tra_lof), testis transformed into ovary from the tra gain of function (XY_tra_gof) and ovary transformed into testis in dsxM gain of function (XX_DsxM_gof/lof) Drosophila melanogaster third instar larvae in biological quadruplicates. In addition, as controls we sequenced ovaries and testes from the female and male wildtype larvae respectively. We constructed polyA+ libraries of the gonads, cleaned off the fatbody and performed 50 bp, stranded single-end RNA-Seq.

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