Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNF4-RGMb axis is required for osteogenic differentiation and cancer cell survival

ABSTRACT: RNF4-RGMb axis is required for osteogenic differentiation and cancer cell survival

PROVIDER: PRJNA845209 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19537647.fastq.gz	Fastqsanger.gz
	SRR19537648.fastq.gz	Fastqsanger.gz
	SRR19537649.fastq.gz	Fastqsanger.gz
	SRR19537650.fastq.gz	Fastqsanger.gz
	SRR19537651.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

RNF4-RGMb axis is required for osteogenic differentiation and cancer cell survival

Project description:Molecular understanding of osteogenic differentiation (OD) of human bone-marrow derived mesenchymal stem cells (hBMSCs) is important for regenerative medicine and has direct implications to cancer. Here we report that the RNF4 ubiquitin ligase is essential for OD of hBMSCs, and that RNF4 deficient hBMSCs fail to differentiate and remain as stalled progenitors. Remarkably, addition of media from differentiating hBMSCs to RNF4-deficient MSCs alleviated the block in OD. Transcriptional analysis of RNF4-dependent gene signature identified two secreted factors; BMP6 and the guidance molecule BMP6 co-receptor RGMb (Dragon). Knockdown of either RGMb or BMP6 in hBMSCs inhibit differentiation, and only the combined addition of purified RGMb and BMP6 proteins to RNF4-deficient MSCs fully restored osteogenic differentiation. Moreover, RNF4-RGMb axis is highly relevant cancer cell survival and tumorigenicity, including osteosarcoma and Therapy-resistant melanoma cells. In accordance, in human melanoma biopsies high level of RGMb correlates with high level of RNF4, and is associated with resistance to molecular therapy and poor prognosis of patients. Thus, RNF4~BMP6~RGMb is a molecular axis that is key for OD and tumorigenesis.

2022-09-28 | GSE205432 | GEO

RNF4~RGMb~BMP6 axis required for osteogenic differentiation and cancer cell survival.

Project description:Molecular understanding of osteogenic differentiation (OD) of human bone marrow-derived mesenchymal stem cells (hBMSCs) is important for regenerative medicine and has direct implications for cancer. We report that the RNF4 ubiquitin ligase is essential for OD of hBMSCs, and that RNF4-deficient hBMSCs remain as stalled progenitors. Remarkably, incubation of RNF4-deficient hBMSCs in conditioned media of differentiating hBMSCs restored OD. Transcriptional analysis of RNF4-dependent gene signatures identified two secreted factors that act downstream of RNF4 promoting OD: (1) BMP6 and (2) the BMP6 co-receptor, RGMb (Dragon). Indeed, knockdown of either RGMb or BMP6 in hBMSCs halted OD, while only the combined co-addition of purified RGMb and BMP6 proteins to RNF4-deficient hBMSCs fully restored OD. Moreover, we found that the RNF4-RGMb-BMP6 axis is essential for survival and tumorigenicity of osteosarcoma and therapy-resistant melanoma cells. Importantly, patient-derived sarcomas such as osteosarcoma, Ewing sarcoma, liposarcomas, and leiomyosarcomas exhibit high levels of RNF4 and BMP6, which are associated with reduced patient survival. Overall, we discovered that the RNF4~BMP6~RGMb axis is required for both OD and tumorigenesis.

| S-EPMC9509360 | biostudies-literature

The neutrophil–osteogenic cell axis promotes bone destruction in periodontitis

Project description:The neutrophil–osteogenic cell axis promotes bone destruction in periodontitis

| PRJNA1071651 | ENA

Sexual dimorphism in osteogenic differentiation

Project description:Sexual dimorphism in osteogenic differentiation

| PRJNA768873 | ENA

Visceral Endoderm differentiation mediated by Hnf1B and is required for epiblast survival

Project description:Visceral Endoderm differentiation mediated by Hnf1B and is required for epiblast survival

| PRJEB49826 | ENA

Sexual dimorphism in osteogenic differentiation

Project description:RNA-sequencing of adult human stem cells determining sexual dimorphisms in osteogenic differentiation.

2022-03-10 | GSE185394 | GEO

HNRNPH1 is required for rhabdomyosarcoma cell growth and survival

Project description:HNRNPH1 is required for rhabdomyosarcoma cell growth and survival

| PRJNA413052 | ENA

MicroRNA profiling of human umbilical cord mesenchymal stem cells during osteogenic differentiation

Project description:The time course experiment was designed as follow: (1) Mesenchymal stem cell (indifferentiated cells)/ time 0h; (2) After 24 h of osteogenic induction; (3) After 48 h of osteogenic induction (4) After 7 days of osteogenic induction.

2009-10-10 | E-MEXP-2259 | biostudies-arrayexpress

Cald1 regulates osteogenic differentiation of PDLSCs.

Project description:Cald1 regulates osteogenic differentiation of PDLSCs.

| PRJNA988422 | ENA

Transcription profiling of human mesenchymal stem cell osteogenic differentiation

Project description:full human genome expression microarrays were used to analysis of gene expression profiles upon the induction of MSC osteogenic differentiation.

2009-05-31 | E-MEXP-2131 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data