Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ATAC-seq to investigate the effect of DGCR8 knockout and heterozygosity DNA accessibility in PA-1 cells

ABSTRACT: ATAC-seq to investigate the effect of DGCR8 knockout and heterozygosity DNA accessibility in PA-1 cells

PROVIDER: PRJNA847617 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19607625_1.fastq.gz	Fastqsanger.gz
	SRR19607625_2.fastq.gz	Fastqsanger.gz
	SRR19607626_1.fastq.gz	Fastqsanger.gz
	SRR19607626_2.fastq.gz	Fastqsanger.gz
	SRR19607627_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

ATAC-seq to investigate the effect of DGCR8 knockout and heterozygosity DNA accessibility in PA-1 cells

Project description:High-throughput profiling to study the effect of DGCR8 heterozygosity on genome-wide chromatin accessibility.

2025-04-02 | GSE205798 | GEO

Effect of DGCR8 knockout and heterozygosity pri-miRNA processing in PA-1 cells

Project description:In this dataset we extract and sequence chromatin-associated RNA to study the effect of DGCR8 knockout and heterozygosity on pri-miRNA processing

2025-02-14 | GSE197469 | GEO

Effect of DGCR8 heterozygosity on steady state RNA levels in embryonic teratocarcinoma PA-1 cells

Project description:In this dataset we use total RNA-seq to study the effect of DGCR8 heterozygosity on steady state RNA levels

2025-02-14 | GSE197472 | GEO

Effect of DGCR8 heterozygosity on steady state miRNA levels in embryonic teratocarcinoma PA-1 cells

Project description:In this dataset we use small RNA-seq to study the effect of DGCR8 heterozygosity on steady state miRNA levels

2025-02-14 | GSE197470 | GEO

Effect of DGCR8 knockout and heterozygosity pri-miRNA processing in PA-1 cells

Project description:Effect of DGCR8 knockout and heterozygosity pri-miRNA processing in PA-1 cells

| PRJNA810449 | ENA

Effect of DGCR8 heterozygosity on steady state RNA levels in embryonic teratocarcinoma PA-1 cells

Project description:Effect of DGCR8 heterozygosity on steady state RNA levels in embryonic teratocarcinoma PA-1 cells

| PRJNA810447 | ENA

Effect of DGCR8 heterozygosity on steady state miRNA levels in embryonic teratocarcinoma PA-1 cells

Project description:Effect of DGCR8 heterozygosity on steady state miRNA levels in embryonic teratocarcinoma PA-1 cells

| PRJNA810448 | ENA

DGCR8 haploinsufficiency leads to primate-specific RNA dysregulation and pluripotency defects

Project description:The 22q11.2 deletion syndrome (22qDS) is caused by a microdeletion in one of the chromosomes 22, comprising around 40 genes and including DGCR8, an essential gene for miRNA biogenesis and transposable elements control. Most of the clinical manifestations of 22qDS have their origin during embryonic development, but the contribution of human DGCR8 hemizygosity to the disease is unknown. To clarify the overall effect of DGCR8 in the context of 22qDS, we have generated two human embryonic models of DGCR8 +/-.In this dataset we use small RNA-seq to study the effect of DGCR8 heterozygosity on steady state miRNA levels in human embryonic stem cells.

2025-02-14 | GSE209771 | GEO

DGCR8 haploinsufficiency leads to primate-specific RNA dysregulation and pluripotency defects

Project description:The 22q11.2 deletion syndrome (22qDS) is caused by a microdeletion in one of the chromosomes 22, comprising around 40 genes and including DGCR8, an essential gene for miRNA biogenesis and transposable elements control. Most of the clinical manifestations of 22qDS have their origin during embryonic development, but the contribution of human DGCR8 hemizygosity to the disease is unknown. To clarify the overall effect of DGCR8 in the context of 22qDS, we have generated two human embryonic models of DGCR8 +/-.In this dataset we use total RNA-seq to study the effect of DGCR8 heterozygosity on steady state RNA levels in human embryonic stem cells.

2025-02-14 | GSE209770 | GEO

Stabilizing Heterochromatin by DGCR8 Alleviates Senescence and Osteoarthritis

Project description:DiGeorge syndrome critical region 8 (DGCR8) is a critical component of the canonical microprocessor complex for microRNA biogenesis. However, the non-canonical functions of DGCR8 have not been studied. Here, we demonstrate that DGCR8 plays an important role in maintaining heterochromatin organization and attenuating aging. An N-terminal-truncated version of DGCR8 (DR8dex2) accelerated senescence in human mesenchymal stem cells (hMSCs) independent of its miRNA-processing activity, which is mediated by its C-terminal domains. Further studies revealed that DGCR8 maintained heterochromatin organization by interacting with the nuclear envelope protein Lamin B1, and heterochromatin-associated proteins, KAP1 and HP1 Overexpression of any of these proteins, including DGCR8, reversed premature senescent phenotypes in DR8dex2 hMSCs. Finally, DGCR8 was downregulated in pathologically and naturally aged hMSCs, whereas DGCR8 overexpression alleviated hMSC aging and osteoarthritis in mice. Taken together, these analyses uncovered a novel, miRNA processing-independent role for DGCR8 in maintaining heterochromatin organization and attenuating senescence. DGCR8 may therefore represent a new therapeutic target for alleviating human aging-related disorders.

2019-05-28 | PXD013856 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data