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Nociceptor neurons affect cancer immunosurveillance [RNA-seq 3]

ABSTRACT: Nociceptor neurons affect cancer immunosurveillance [RNA-seq 3]

PROVIDER: PRJNA847968 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19619843.fastq.gz	Fastqsanger.gz
	SRR19619844.fastq.gz	Fastqsanger.gz
	SRR19619845.fastq.gz	Fastqsanger.gz
	SRR19619846.fastq.gz	Fastqsanger.gz
	SRR19619847.fastq.gz	Fastqsanger.gz

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Nociceptor neurons affect cancer immunosurveillance

Project description:Nociceptor neurons affect cancer immunosurveillance

| PRJNA847963 | ENA

Nociceptor neurons affect cancer immunosurveillance [RNA-seq 2]

Project description:Nociceptor neurons affect cancer immunosurveillance [RNA-seq 2]

| PRJNA847969 | ENA

Nociceptor neurons affect cancer immunosurveillance [RNA-seq 1]

Project description:Nociceptor neurons affect cancer immunosurveillance [RNA-seq 1]

| PRJNA847967 | ENA

Nociceptor neurons affect cancer immunosurveillance.

Project description:Solid tumours are innervated by nerve fibres that arise from the autonomic and sensory peripheral nervous systems1-5. Whether the neo-innervation of tumours by pain-initiating sensory neurons affects cancer immunosurveillance remains unclear. Here we show that melanoma cells interact with nociceptor neurons, leading to increases in their neurite outgrowth, responsiveness to noxious ligands and neuropeptide release. Calcitonin gene-related peptide (CGRP)-one such nociceptor-produced neuropeptide-directly increases the exhaustion of cytotoxic CD8+ T cells, which limits their capacity to eliminate melanoma. Genetic ablation of the TRPV1 lineage, local pharmacological silencing of nociceptors and antagonism of the CGRP receptor RAMP1 all reduced the exhaustion of tumour-infiltrating leukocytes and decreased the growth of tumours, nearly tripling the survival rate of mice that were inoculated with B16F10 melanoma cells. Conversely, CD8+ T cell exhaustion was rescued in sensory-neuron-depleted mice that were treated with local recombinant CGRP. As compared with wild-type CD8+ T cells, Ramp1-/- CD8+ T cells were protected against exhaustion when co-transplanted into tumour-bearing Rag1-deficient mice. Single-cell RNA sequencing of biopsies from patients with melanoma revealed that intratumoral RAMP1-expressing CD8+ T cells were more exhausted than their RAMP1-negative counterparts, whereas overexpression of RAMP1 correlated with a poorer clinical prognosis. Overall, our results suggest that reducing the release of CGRP from tumour-innervating nociceptors could be a strategy to improve anti-tumour immunity by eliminating the immunomodulatory effects of CGRP on cytotoxic CD8+ T cells.

| S-EPMC9646485 | biostudies-literature

Nociceptor neurons affect cancer immunosurveillance [RNA-seq 3]

Project description:Sequecing tumor-innervating neurons

2022-08-10 | GSE205865 | GEO

Nociceptor neurons affect cancer immunosurveillance

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-08-10 | GSE205866 | GEO

TUMOR-INFILTRATING NOCICEPTOR NEURONS PROMOTE IMMUNOSUPPRESSION

Project description:Nociceptor neurons impact tumor immunity. Removing nociceptor neurons reduced myeloid-derived suppressor cell (MDSCs) tumor infiltration in mouse models of head and neck carcinoma and melanoma. Carcinoma-released small extracellular vesicles (sEVs) attract nociceptive nerves to tumors. sEV-deficient tumors fail to develop in mice lacking nociceptor neurons. Exposure of dorsal root ganglia (DRG) neurons to cancer sEVs elevated expression of Substance P, IL-6 and injury-related neuronal markers while treatment with cancer sEVs and cytotoxic CD8 T-cells induced an immunosuppressive state (increased exhaustion ligands and cytokines). Cancer patient sEVs enhanced DRG responses to capsaicin, indicating increased nociceptor sensitivity. Conditioned media from DRG and cancer cell co-cultures promoted expression of MDSC markers in primary bone marrow cells while DRG conditioned media together with cancer sEVs induced checkpoint expression on T-cells. Our findings indicate that nociceptor neurons facilitate CD8+ T cell exhaustion and enhance MDSC infiltration. Targeting nociceptor-released IL-6 emerges as a novel strategy to disrupt harmful neuro-immune interactions in cancer and enhance anti-tumor immunity.

2025-08-07 | GSE300254 | GEO

TUMOR-INFILTRATING NOCICEPTOR NEURONS PROMOTE IMMUNOSUPPRESSION [MDSC]

2025-08-07 | GSE300787 | GEO

Nociceptor neurons affect cancer immunosurveillance [RNA-seq 2]

Project description:Sequencing Neuron, melanoma and CD8 when culture alone or co-culture with one another

2022-08-10 | GSE205864 | GEO

Nociceptor neurons affect cancer immunosurveillance [RNA-seq 1]

Project description:Neuron, melanoma and CD8 co-cultures

2022-08-10 | GSE205863 | GEO