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Effect of SOX9 depletion on active chromatin marks in hESC-derived cranial neural crest cells [ChIP-seq]

ABSTRACT: Effect of SOX9 depletion on active chromatin marks in hESC-derived cranial neural crest cells [ChIP-seq]

PROVIDER: PRJNA848206 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19630265_1.fastq.gz	Fastqsanger.gz
	SRR19630265_2.fastq.gz	Fastqsanger.gz
	SRR19630266_1.fastq.gz	Fastqsanger.gz
	SRR19630266_2.fastq.gz	Fastqsanger.gz
	SRR19630267_1.fastq.gz	Fastqsanger.gz

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Effect of SOX9 depletion on active chromatin marks in hESC-derived cranial neural crest cells [ChIP-seq]

Project description:To assess the effects of SOX9 depletion on active chromatin marks in hESC-derived cranial neural crest cells, we performed genome editing of the H9 hESC lines to tag SOX9 with FKBPV36, V5, and mNeonGreen. Upon differentiating edited hESC to cranial neural crest cells using an established protocol, addition of the degrader molecule dTAGV-1 results in SOX9 depletion. We then profiled the active chromatin mark H3K27ac by ChIP-Seq in undepleted CNCCs or CNCCs in which SOX9 was depleted for 3h or 24h. We also titrated SOX9 to four distinct levels with dTAGV-1 and assessed SOX9 binding by V5 ChIP-seq, as well as TWIST1 and TFAP2A binding by ChIP-seq.

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Project description:Effect of SOX9 dosage changes on chromatin accessibility in hESC-derived cranial neural crest cells [ATAC-seq]

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