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Convergence of distinct RNA-silencing pathways on GW182/Tnrc6 [RNA-seq]

ABSTRACT: Convergence of distinct RNA-silencing pathways on GW182/Tnrc6 [RNA-seq]

PROVIDER: PRJNA848669 | ENA |

REPOSITORIES: ENA

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Convergence of distinct RNA-silencing pathways on GW182/Tnrc6

Project description:Convergence of distinct RNA-silencing pathways on GW182/Tnrc6

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Convergence of distinct RNA-silencing pathways on GW182/Tnrc6 II

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Convergence of distinct RNA-silencing pathways on GW182/Tnrc6

Project description:The RNA-binding protein Trim71/Lin41 is a phylogenetically conserved developmental regulator that functions in mammalian stem cell reprogramming, brain development and cancer. Trim71 recognizes target mRNAs through hairpin motifs and silences them through molecular mechanisms that await identification. Deposited data are mass spectrometry data from immunoprecipitation experiments with endogenously tagged Trim71, Ago2, and Tnrc6a. Experiments are carried out in mouse embryonic stem cells (mESCs) and uncover reciprocal interactions of Trim71, Ago2, and Tnrc6a. Trim71 protein interactions are largely independent of Ago2 levels, but strongly depend on the presence of RNA. A second set of experiments is mass spectrometry data from immunoprecipitation experiments in mESCs overexpressing a tagged peptide derived from Homo sapiens TNRC6B (‘FLAG-HA::T6BWT::Cherry’), that is known to block the Tnrc6-Ago2 interaction. We show that a wild-type version strongly binds Ago1 and Ago2, but not Trim71, while a mutant version binds neither Ago1, 2, nor Trim71.

2023-07-04 | PXD034537 | Pride

Convergence of distinct RNA-silencing pathways on GW182/Tnrc6

2023-07-04 | PXD041242 | Pride

Convergence of distinct RNA-silencing pathways on GW182/Tnrc6 [sgRNA screen]

Project description:sgRNA screens for factors influencing Trim71 silencing activity.

2023-06-26 | GSE214708 | GEO

Convergence of distinct RNA-silencing pathways on GW182/Tnrc6 II

Project description:RNA-sequencing in mouse embryonic stem cells: Ctrl: GFP_5xT71BS (mES [129S6/SvEvTac], yGlob [GFP_5xT71BS]. Trim71-/-: GFP_5xT71BS (mES [129S6/SvEvTac], yGlob [GFP_5xT71BS] Trim71 -/-. Note pervasive gene expression changes in samples with TNRC6A overexpression in comparison with samples with CAS9 overexpression.

2024-02-13 | GSE231730 | GEO

miR-122-dependent binding of TNRC6 to HCV RNA

Project description:The liver-specific microRNA, miR-122, is an essential host factor for replication of hepatitis C virus (HCV), an important infectious cause of chronic liver disease and hepatocellular carcinoma. miR-122 stabilizes the positive-strand HCV RNA genome and promotes viral RNA synthesis by binding two closely spaced sites (S1 and S2) near the 5’ end of the genome in association with Ago2. Ago2 is essential for both host factor activities, but whether other host proteins are involved is unknown. Using a quantitative proteomics approach, we identified TNRC6A (GW182) and its paralogs (TNRC6B and TNRC6C), as functionally important components of the miR-122/Ago2 host factor complex binding HCV RNA. Depletion of any two TNRC6 proteins reduced HCV replication in Huh-7.5 cells,but did not reduce viral RNA stability or translational activity, but rather dampened miR-122 stimulation of viral RNA synthesis. However, TNRC6 depletion had no effect on replication of HCV in which S2 was mutated so that miR-122 binds only S1, whereas it significantly enhanced replication when S1 was mutated and only S2 bound by miR-122. Consistent with this, we found that TNRC6 proteins preferentially associate with the S1 site, and that the association of Ago2 with S2 is increased in TNRC6-depleted cells. Collectively, these data suggest a model in which TNRC6 proteins, which are known to interact with Ago2, preferentially direct the miR-122/Ago2 complex to S1 while restricting its association with S2, thereby fine tuning the spatial organization of miR-122/Ago2 complexes bound to the viral RNA.

2019-04-24 | PXD013054 | Pride

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