Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Toosendanin suppresses African swine fever virus replication through upregulating IRF1 in porcine alveolar macrophage cultures

ABSTRACT: Toosendanin suppresses African swine fever virus replication through upregulating IRF1 in porcine alveolar macrophage cultures

PROVIDER: PRJNA849226 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19654616_1.fastq.gz	Fastqsanger.gz
	SRR19654616_2.fastq.gz	Fastqsanger.gz
	SRR19654617_1.fastq.gz	Fastqsanger.gz
	SRR19654617_2.fastq.gz	Fastqsanger.gz
	SRR19654618_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Toosendanin suppresses African swine fever virus replication through upregulating IRF1 in porcine alveolar macrophage cultures

Project description:African swine fever virus (ASFV) is a highly infectious and lethal swine pathogen that causes severe socio-economic consequences in affected countries. Unfortunately, effective vaccine for combating ASF is unavailable so far, and the prevention and control strategies for ASFV are still very limited. Toosendanin (TSN), a triterpenoid saponin extracted from the medicinal herb Melia toosendan Sieb. Et Zucc, has been demonstrated to possess analgesic, anti-inflammatory, anti-botulism and anti-microbial activities, and was used clinically as an anthelmintic, while the antiviral effect of TSN on ASFV has not been reported. In this study, we revealed that TSN exhibited a potent inhibitory effect on ASFV GD955-38 strain in porcine alveolar macrophages (PAMs) (EC50=0.085 μM, SI = 365) in a dose-dependent manner. TSN showed robust antiviral activity in different doses of ASFV infection and reduced the transcription and translation levels of ASFV p30 protein, viral genomic DNA quantity as well as viral titer at 24 and 48 hours post-infection. In addition, TSN did not affect virion attachment and release but intervened in its internalization in PAMs. Further investigations disclosed that TSN played its antiviral role by upregulating the host IFN-stimulated gene (ISG) IRF1 rather than by directly inactivating the virus particles. Overall, our results suggest that TSN is an effective antiviral agent against ASFV replication in vitro and may have the potential for clinical use.

2022-08-08 | GSE206118 | GEO

African swine fever virus

Project description:African swine fever virus overview

| PRJNA69653 | ENA

African swine fever virus

Project description:African swine fever virus Genome sequencing

| PRJNA776429 | ENA

African swine fever virus

Project description:African Swine Fever Virus Genome Sequencing

| PRJNA983205 | ENA

African swine fever virus

Project description:African swine fever virus Genome sequencing