Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile at single cell level of thymic stromal cells

ABSTRACT: Gene expression profile at single cell level of thymic stromal cells

PROVIDER: PRJNA851030 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Gene expression profile at single cell level of thymic stromal cells

Project description:Thymic atrophy and the progressive immune decline that accompanies it is a major health problem, chronically with age and acutely with immune injury. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of mesenchymal niche cells (ThyMC) in human and murine thymi.

2025-09-09 | GSE206459 | GEO

A single cell transcriptome atlas of Human thymic stromal compartment

Project description:A single cell transcriptome atlas of Human thymic stromal compartment

| PRJNA670375 | ENA

Gene expression profile at single cell level of iPSC-derived and primary thymic epithelial cells (TECs)

Project description:Gene expression profile at single cell level of iPSC-derived and primary thymic epithelial cells (TECs)

| PRJNA1154010 | ENA

Global transcriptional profiling reveals distinct functions of thymic stromal subsets and age-related changes during thymic involution

Project description:The thymic microenvironment is essential for proper differentiation and selection of thymocytes.Thymic involution in aged mice results in decreased T cell output and immune function. Here we use gene expression profiling of FACS sorted thymic stromal subsets to identify molecular mediators of thymocyte: stromal cell interactions, as well as gene expression changes thymic stromal subsets during early stages of thymic involution . We used microarrays to analyze gene expression differences between thymic stromal subsets from male C57BL/6J mice 1, 3, and 6 months of age. Thymic stromal subsets (cTEC, mTEClo, mTEChi, Sirpa-DC, Sirpa+DC, and fibroblasts) were isolated from two 1-, 3-, and 6- month old male C57BL/6J mice. After enzymatic digestion of the thymi, the stromal cells were FACS purified, and RNA was extracted, amplified, labeled and hybridized to Affymetrix mouse 430 2.0 arraysarrays. Raw data were uploaded to Gene Expression Commons for normalization. Both raw CEL and normalized datasets from the 36 samples are included. A model within Gene Expression Commons has been created for analyses/comparisons of these datasets, along with previously reported thymocyte subset datasets. The model within Gene Expression Commons thus contains 6 thymic stromal populations, each from mice 1, 3, and 6 months of age, with duplicates for each datset.

2014-09-12 | E-GEOD-56928 | biostudies-arrayexpress

Global transcriptional profiling reveals distinct functions of thymic stromal subsets and age-related changes during thymic involution

2014-09-12 | GSE56928 | GEO

Gene expression profile at single cell level of donor-matched primary bovine Mesenchymal Stromal Cells (MSCs)

Project description:Gene expression profile at single cell level of donor-matched primary bovine Mesenchymal Stromal Cells (MSCs)

| PRJNA1009209 | ENA

MRAP mediated adipocyte differentiation by thymic mesenchymal stromal cells contributes to thymic involution

Project description:Adipocyte deposition is believed to be a primary characteristic of age-related thymic involution, but the underlying cellular and molecular mechanisms remain unknown. We show here that thymic mesenchymal stromal cells (tMSCs) have a higher tendency to differentiate into adipocytes and melanocortin-2 receptor accessory protein (MRAP) is a potential driver of tMSCs adipogenesis. Furthermore, we discover that thymosin-α1 promotes MRAP expression in tMSCs through FoxO1 signaling pathway. Additionally, the proportion of tMSCs increase in older mice compared to young mice. Importantly, MRAP is also necessary for human thymic MSCs to differentiate into adipocytes when exposed to thymosin-α1. Single-cell RNA-seq analysis of human thymus revealed an accumulation of tMSCs and adipocytes during aging, indicating a strong potential for adipogenic differentiation in age-related thymic involution. Thus, we have revealed MRAP as a key factor in promoting thymic MSCs adipogenesis triggered by thymosin-α1 and FoxO1 pathway, which may serve as potential target to hinder adiposity in age-related thymic involution.

2024-11-27 | GSE183261 | GEO

Gene expression profile at single cell level of stromal cells(SCs) from the periosteum and bone marrow

Project description:Gene expression profile at single cell level of stromal cells(SCs) from the periosteum and bone marrow

| PRJNA1049438 | ENA

RNA-seq analysis on thymic stromal cells

Project description:RNA-seq analysis on thymic stromal cells

| PRJNA614004 | ENA

Gene expression profile at single cell level of thalamus

Project description:Gene expression profile at single cell level of thalamus

| PRJNA942935 | ENA