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Single cell RNA-sequencing of vehicle- and UK5099-treated mouse prostate organoids

ABSTRACT: Single cell RNA-sequencing of vehicle- and UK5099-treated mouse prostate organoids

PROVIDER: PRJNA851368 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19796049_1.fastq.gz	Fastqsanger.gz
	SRR19796049_2.fastq.gz	Fastqsanger.gz
	SRR19796050_1.fastq.gz	Fastqsanger.gz
	SRR19796050_2.fastq.gz	Fastqsanger.gz

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Project description:The goal of this study was to perform transcriptomics on vehicle-, UK5099-, Butyrate-, and UK5099+Butyrate-treated mouse prostate organoids. We used FACS to isolate basal cells from C57BL/6 mouse prostates and treated with small molecules for one week before harvesting for RNA-sequencing.

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Single cell RNA-sequencing of vehicle- and UK5099-treated mouse prostate organoids

Project description:The goal of this study was to determine how modulation of mitochondrial pyruvate metabolism regulates gene expression in a three-dimensional organoid model of mouse prostate epithelium. Organoids were treated with the small molecule UK5099, which impairs mitochondrial pyruvate uptake, and subjected to single-cell RNA-sequencing. Our analysis revealed considerable heterogeneity within prostate organoids characterized by distinct cell clusters expressing markers associated with basal, luminal and mesenchymal-like cells. UK5099 treatment led to a reduction in epithelial populations and an enrichment in mesenchymal-like cells. These findings indicate that pyruvate metabolism can modulate features of cell fate in prostate epithelium.

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Project description:RNA sequencing of mouse basal-derived prostate organoids treated with vehicle, UK5099, Butyrate, or UK5099+Butyrate

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Project description:The goal of this study was to understand how the mitochondrial pyruvate carrier inhibitor,UK5099, and lactate supplementation alter chromatin accessibility in primary basal-derived mouse prostate organoids.

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