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Integration of single-cell transcriptomes and biological function reveals distinct behavioral patterns in bone marrow endothelial cells [bulk RNA-seq]

ABSTRACT: Integration of single-cell transcriptomes and biological function reveals distinct behavioral patterns in bone marrow endothelial cells [bulk RNA-seq]

PROVIDER: PRJNA853187 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19859200.fastq.gz	Fastqsanger.gz
	SRR19859201.fastq.gz	Fastqsanger.gz
	SRR19859202.fastq.gz	Fastqsanger.gz
	SRR19859203.fastq.gz	Fastqsanger.gz
	SRR19859204.fastq.gz	Fastqsanger.gz

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Integration of single-cell transcriptomes and biological function reveals distinct behavioral patterns in bone marrow endothelial cells

Project description:Integration of single-cell transcriptomes and biological function reveals distinct behavioral patterns in bone marrow endothelial cells

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Integration of single-cell transcriptomes and biological function reveals distinct behavioral patterns in bone marrow endothelium.

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Integration of single-cell transcriptomes and biological function reveals distinct behavioral patterns in bone marrow endothelial cells

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Integration of single-cell transcriptomes and biological function reveals distinct behavioral patterns in bone marrow endothelial cells [scRNA-Seq]

Project description:Heterogeneity of endothelial cell populations reflects their diverse functions in maintaining tissue’s homeostasis. However, their phenotypic, molecular, and functional properties are not entirely mapped. We used a new Tie2-CreERT2;Rosa26-tdTomato reporter mouse to trace, profile, and cultivated primary ECs from different organs. As platform paradigm, we used this strategy to study bone marrow endothelial cells (BMEC). Single-cell mRNA sequencing of primary BMEC revealed that their diversity and native molecular signatures is transitorily preserved in an ex-vivo culture that conserves key cell-to-cell microenvironment interactions. Macrophages sustained primary BMEC cellular diversity and expansion and were critical to preserve sinusoidal-like BMEC ex-vivo. Expression of endomucin discriminated BMEC in populations exhibiting mutually exclusive properties and distinct tip and stalk signatures. In contrast to arterial-like, sinusoidal-like BMECs were short-lived, formed 2D-networks, contributed to in-vivo angiogenesis and supported hematopoietic stem/progenitor cells in-vitro. This platform can be extended to other organs’ ECs to decode mechanistic information and explore therapeutics.

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