Project description:Here we determine the impact upon the loss of M66 dsRNA alone and both L-A-66 along with M66 on the host S. paradoxus transcriptional changes. In Pet [L+M-] and [L-M-] cells we have found 941 and 651 differently expressed genes (DEGs), respectively. In total, there were 1234 and 535 DEGs in Spo [L+M-] and [L-M-] cells, respectively.
Project description:We assigned carboxypeptidase X 2 (Cpxm2) to a genetic locus for left ventricular mass. The functional role of Cpxm2 was investigated in Cpxm2-deficient (KO) and wild-type (WT) mice exposed to deoxycorticosterone acetate (DOCA)-salt hypertension and control conditions (SHAM). Both WT and KO animals developed severe and similar systolic hypertension in response to DOCA. WT mice developed severe LV damage. These changes were significantly ameliorated or even normalized (i.e. ejection fraction) in KO-DOCA animals. LV transcriptome analysis in WT, but not in KO mice, showed a molecular cardiac hypertrophy/remodeling signature with significant upregulation of 1234 transcripts including Cpxm2 in response to DOCA.