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To find target miRNA of Zeb1

ABSTRACT: To find target miRNA of Zeb1

PROVIDER: PRJNA853954 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19896033.fastq.gz	Fastqsanger.gz
	SRR19896034.fastq.gz	Fastqsanger.gz
	SRR19896035.fastq.gz	Fastqsanger.gz
	SRR19896036.fastq.gz	Fastqsanger.gz

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To find target miRNA of Zeb1

Project description:To investigate potential target miRNA of Zeb1 that were involved in regulation of antigen cross-presentation. We then performed gene expression profiling analysis using data obtained from miRNA-seq from WT and Zeb1-deficient BMDC after HKLM-OVA stimulating for 4 hours.

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To find target genes of Zeb1 [CUT&Tag-Seq]

Project description:To find target genes of Zeb1 [CUT&Tag-Seq]

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To find target genes of Zeb1 [CUT&Tag-Seq]

Project description:To investigate potential target genes of Zeb1 that were involved in regulation of antigen cross-presentation. We then performed CUT&tag about Zeb1 gene from WT BMDC after HKLM-OVA stimulating for 4 hours

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Target suppression effect by methylated miRNA

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3’ Uridylation Expands miRNA Target Repertoire

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Effect of depletion of Zeb1 in CD11c+ dendritic cells

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Profiling of microRNA expression in lung cancer cells with up-regulation of Zeb1

Project description:The Zeb1 transcriptional repressor plays a key role in metastasis through the down-regulation of genes that are strong inducers of epithelial differentiation and inhibitors of stem-ness. Here we report that Zeb1 controls the expression of numerous oncogenic and tumor suppressive microRNAs (miRs). Zeb1 stimulated pro-migratory cytoskeletal processes by down-regulating miR-34a and activated Rho GTPases through Arhgap1, a Cdc42 GTPase activating protein and novel miR-34a target gene. Poor-prognosis human lung adenocarcinomas were highly enriched in a cytoskeletal gene signature activated by miR-34a down-regulation. These findings suggest that Zeb1 regulates a miR network and drives pro-migratory cytoskeletal processes through miR-34a.

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ChIP-seq with anti-ZBTB17 antibody in HeLa cells to find target genes

Project description:ChIP-seq with anti-ZBTB17 antibody in HeLa cells to find target genes

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microRNA-seq analysis studying the role of ZEB1 in Th0 and Th17 differentiation [miRNA-seq]

Project description:We performed microRNA-seq to study the role of ZEB1 in human Th17 cells differentiation.

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Impact of ectopic expression of SNAIL2, ZEB2, ZEB1 or TWIST1 on BRAF-target genes in the murine melanocytic melan-a cell line

Project description:We have demonstrated that the oncogenic activation of B-RAF (using a truncated delta-BRAF-ER version inducible with tamoxifen) in the melan-a melanocyte cell line triggers the activation of Zeb1 and Twist1 at the expanse of Zeb2 and Snail2. Enforced maintenance of Zeb2 or Snail2 expression reduces the B-RAF oncogenic potential while ectopic expression of Zeb1 or Twist1 cooperates with B-RAF in melan-a cell transformation. To get an insight into the properties of these embryonic transcription factors, gene expression profiles of melan-a-derived cell lines either expressing a non-activated B-RAF (- tamoxifen) or an activated BRAF (+ tamoxifen) alone or in combination with Snail2, Zeb2, Twist1 or Zeb1 have been established. Melan-a cells were transduced with an activated version of BRAF(delta-BRAF-ER, inducible with tamoxifen) alone or in combination with SNAIL2, ZEB2, ZEB1 or TWIST1. Gene expression profiles before or following BRAF activation (alone or in combination with the embryonic transcription factors) were determined. Ectopic expression of SNAIL2, ZEB2, ZEB1 or TWIST1 on BRAF-target genes in the murine melanocytic melan-a cell line.

2013-10-29 | E-GEOD-39030 | biostudies-arrayexpress

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