Transcriptomics analysis of dsu and WT response to infection of U. maydis.
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA854090 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR19897810_1.fastq.gz | Fastqsanger.gz | |||
| SRR19897810_2.fastq.gz | Fastqsanger.gz | |||
| SRR19897811_1.fastq.gz | Fastqsanger.gz | |||
| SRR19897811_2.fastq.gz | Fastqsanger.gz | |||
| SRR19897812_1.fastq.gz | Fastqsanger.gz |
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Project description:To investigate the different responses of WT and dsu to U. maydis, transcriptome analysis was perfromed and the results showed that reprogramming of maize transcription after infection U. maydis.
2022-07-02 | GSE207185 | GEO
Project description:The biotrophic fungal pathogen Ustilago maydis cause common smut in maize, and lead to gall formation on all aerial organs, especially on maize kernel thus reduce yield. The interaction of U. maydis with maize is a well-established model to study the interaction between maize and biotrophic pathogen. U. maydis infection could activate host immune responses including: ROS accumulation, protease activation, salicylic acid signaling. U. maydis employ several strategies to overcome maize immune response, thus initial the biotrophic interaction with host. It has been suggested that genetic factors of maize host affected the disease severity of U. maydis infection, here we investigated the transcriptome profile of resistance and susceptible maize lines upon U. maydis infection, thus propose candidate maize genes involved in the defense response in maize to corn smut cause by U. maydis.
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Project description:The fungal pathogen Ustilago maydis establishes a biotrophic relationship with its host plant maize. Hallmarks of the disease are large plant tumors in which fungal proliferation occurs. Plants have developed various defense pathways to cope with pathogens. We used microarrays to detail the global programme of gene expression during the infection process of Ustilago maydis in its host plant to get insights into the defense programs and the metabolic reprogramming needed to supply the fungus with nutrients. Experiment Overall Design: In three independent experiments plants were infected with the solopathogenic U. maydis strain SG200. Samples from infected leaves were taken at 12 and 24 hours post infection, as well as 2, 4 and 8 days post infection. Samples from uninfected control plants were taken at the same time points.
2008-04-07 | E-GEOD-10023 | biostudies-arrayexpress
Project description:Anthocyanin induction in plant is considered a general defense response against biotic and abiotic stresses. The infection by Ustilago maydis, the corn smut pathogen, is accompanied with anthocyanin induction in leaf tissue. We revealed that anthocyanin is intentionally induced by the virulence promoting secreted effector protein Tin2. Tin2 protein functions inside plant cells where it interacts with cytoplasmic maize protein kinase ZmTTK1. Tin2 masks an ubiquitin-proteasome degradation motif in ZmTTK1 leading to a more stable active kinase. Active ZmTTK1 controls transcriptional activation of genes in the anthocyanin biosynthesis pathway rerouting phenylalanine away from lignin biosynthesis. Therefore, we performed microarray analysis to understand how maize gene transcription in phenylpropanoid pathway is differentially changed after infection with Ustilago maydis SG200 (wild type) and SG200Dtin2 (anthocyanin-inducing effector mutant).
2014-01-28 | GSE48536 | GEO
Project description:The fungal pathogen Ustilago maydis establishes a biotrophic relationship with its host plant maize. Hallmarks of the disease are large plant tumors in which fungal proliferation occurs. Plants have developed various defense pathways to cope with pathogens. We used microarrays to detail the global programme of gene expression during the infection process of Ustilago maydis in its host plant to get insights into the defense programs and the metabolic reprogramming needed to supply the fungus with nutrients. Keywords: time course
2008-03-01 | GSE10023 | GEO
Project description:Elicidate the changes of the transcriptional program response of U. maydis WT strain (a2b2) when transfered from neutrality to acidic or alkaline medium The mutants strains CL211 and GP25 used as controls as well as the WT strain are described in Martínez-Espinoza et al., 1997; 2004;González-Prieto et al., 2014 and Banuett & Herskowitz, 1989 respectively.
2016-02-23 | GSE75053 | GEO
Project description:Elicidate the changes of the transcriptional program response of U. maydis WT strain (a2b2) when transfered from neutrality to acidic or alkaline medium The mutants strains CL211 and GP25 used as controls as well as the WT strain are described in MartÃnez-Espinoza et al., 1997; 2004;González-Prieto et al., 2014 and Banuett & Herskowitz, 1989 respectively. High density-one color arrays from Nimblegen were used according to a design from Scott Gold from University of Georgia. The design has five different oligonucleotides of 60 bp in lenght in duplicate, representing each one of the 6883 gene of the U. maydis genome.
2016-02-23 | E-GEOD-75053 | biostudies-arrayexpress
Project description:In this study, RNA-seq based comparative transcriptome analysis was used to study the response between Fusarium graminearum and Ustilago maydis to different growth conditions. RNA-seq libraries were generated from fungal filaments growing in culture (complete medium) and from infected maize silk. This data set contains the data for the Fusarium graminearum and Ustilago maydis medium growth condition.
2019-03-17 | E-MTAB-4562 | biostudies-arrayexpress
Project description:The basidiomycete Ustilago maydis causes smut disease in maize. Colonization of the host plant is initiated by direct penetration of cuticle and cell wall of maize epidermis cells. The invading hyphae are surrounded by the plant plasma membrane and proliferate within the plant tissue. We identified a novel secreted protein, termed Pep1. Disruption mutants of pep1 are not affected in saprophytic growth and develop normal infection structures. However, Îpep1 mutants fail to penetrate the epidermal cell wall and elicit a strong plant defense response. Using Affymetrix maize arrays we identified about 110 plant genes which are differentially regulated in Îpep1 and wild type infections during the penetration stage. Experiment Overall Design: In three independent experiments plants were infected with the strain SG200Dpep1 which is derived from the solopathogenic U. maydis strain SG200. Samples from infected leaves were taken at 24 hours post infection. Samples were treated under the same conditions as described previosly (Doehlemann et al. (2008) Plant J, in press).
2008-10-25 | E-GEOD-12892 | biostudies-arrayexpress
Project description:The infection process is characterized by the simultaneous production of metabolites by both the pathogen and the host. Recently, it has been discovered that molecules generated during sulfur metabolism, mainly hydrogen sulfide (H₂S), play a key role in regulating which organism will prevail during pathogenesis. This phenomenon has been described in bacterial and filamentous fungi infections. Ustilago maydis is a saprophytic fungus that infects maize plants and causes crop losses on a global scale. Although the role of various biomolecules produced by U. maydis during the infection process has been described (ustilagic acid, polyketides, and lipids among others), the function of sulfur-containing compounds, like cysteine and H₂S in this process remains uncharacterized. Here, U. maydis was incubated in a culture medium that promotes H₂S production. The cytoplasmic extract was then obtained, and proteins were extracted and enzymatically digested with trypsin. The resulting peptides were separated using a UPLC NanoAcquity M-Class system. The spectra of these peptides were acquired using MS with electrospray ionization (ESI), employing a data-independent acquisition (DIA) approach and multiplexed high-definition tandem mass spectrometry (HD-MS/MS). Thus, a total of 1,706 proteins were identified and quantified using an internal standard and the intensity of the most abundant peptides, analyzed with Progenesis QI software v4.2.1 and an Ustilago maydis FASTA database (downloaded from UniProt, UP000000561, containing 6,805 protein sequences). Among the identified proteins, 290 were upregulated, while 160 were downregulated. Upon incubation in the aforementioned medium, proteins related to mitochondrial respiration, proteasomal protein degradation, and fatty acid metabolism were upregulated, whereas proteins associated with oxidative stress response, the pentose phosphate pathway (PPP), and translation were downregulated. Additionally, an increase in proteins related to the infectious process, such as endoxylanases and proteinases, was also observed. Thus, this study describes for the first time the role of cysteine metabolism regulation in proteome dynamics in Ustilago maydis.
2025-03-13 | PXD060479 | Pride