Project description:This SuperSeries is composed of the following subset Series: GSE13478: Pearl millet seedlings treated with methyl jasmonate (MeJA) GSE13479: Pearl millet seedlings infected with rust (Puccinia substriata) GSE13480: Pearl millet seedlings treated with salicylic acid (SA) Refer to individual Series
Project description:We analysed global gene expression in the primary root tip of 2 pearl millet (Pennisetum glaucum) inbred lines with high (line 249) and low (line 337) primary root growth using RNAseq. The objective was to identify genes potentially associated with changes in root growth.
Project description:We analyzed global gene expression in the crown tip of 2 pearl millet (Pennisetum glaucum) inbred lines with high (line 249) and low (line 220) root soil aggregation using RNAseq. The obtective was to identify genes potentially associated with changes in rhizosheath formation.
Project description:Pearl millet (Pennisetum glaucum) is a cereal crop well-adapted to arid and high-temperature environments. Its root system exhibits a high degree of complexity, comprising distinct root types with specific anatomical and functional characteristics. These include the primary root, which is established during embryogenesis; crown roots, which develop at the base of the stem post-embryonically; and lateral roots, which emerge from both the primary and crown roots. In this study, we conducted a comparative analysis of gene expression profiles across these distinct root types to better understand their functional specialization.
Project description:Pearl millet is a major cereal crop that feeds more than 90 million people worldwide in arid and semi-arid regions. The stalk phenotypes of Poaceous grasses are critical for their productivity and stress tolerance, however, the molecular mechanisms governing stalk development in pearl millet remained to be deciphered. In this study, we spatiotemporally measured 19 transcriptomes for stalk internodes of four different early developmental stages. Data analysis of the transcriptomes defined 4 developmental zones on the stalks and identified 12 specific gene sets with specific expression patterns across the zones. Using weighted gene co-expression network analysis (WGCNA), we found that 2 co-expression modules together with candidate genes were involved in stalk elongation and thickening of pearl millet. Among the elongation-related candidate genes, we established by SELEX that a MYB-family transcription factor PMF7G02448 can bind to the promoters of three cell wall synthases genes (CesAs). In summary, these findings provide insights into stalk development and offer potential targets for future genetic improvement of pearl millet.
Project description:Transcriptional profiling of rust-infected pearl millet seedlings over time [0h, 20h, 5d and 8d post infection (pi)]. Keywords: Time course, Stress response
Project description:Transcriptional profiling of SA-treated pearl millet seedlings over time [0, 12, 24 and 48 hours post treatment (hpt)]. Keywords: Time course, Stress response
Project description:Transcriptional profiling of MeJA-treated pearl millet seedlings over time [0, 12, 24 and 48 hours post treatment (hpt)]. Keywords: Time course, Stress response
Project description:The marker- and genome-based development of drought resistant and high yield cereal crops is the most pressing activity in a constantly more stress- and harmful environment for plant productivity. Genome-based assisted breeding is only capable to cover 30-40 % of phenotypic variance according to the most recent GWAS studies. There are many processes which are not predictable by genome information, especially protein translation and activity which is crucial for phenotypic responses and survival of the plants under severe stresses. Therefore, we present comparative proteomic and physiological analysis under drought stress in two of the most important staple food crops pearl millet and wheat thereby representing C4 and C3 plants. Here, we have selected contrasting genotypes, and performed a large-scale comparative analysis from the molecular to the phenotypic level under drought stress. We were able to establish molecular-physiological phenotypes for: 1. Stay green protein signature in contrasting pearl millet genotypes which is highly correlated to the physiological data, in the submitted manuscript. 2. No clear indication of stay green proteome signatures in contrasting wheat genotypes but instead differential senescence proteome signatures not capable to cope with similar drought stress. These mechanisms are decisive for drought resistance and yield/grain filling under stress conditions and for the first time these physiological phenotypes (seed yield, root growth, and photosynthesis) are directly linked to the molecular proteomic phenotype. We think these results are of broad importance for the scientific community. This study demonstrates the enormous molecular and phenotypic plasticity because the selected genotypes represent the extreme points of stress adaptation and yield protection.
