Ontology highlight
ABSTRACT:
PROVIDER: PRJNA856798 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR20045315_1.fastq.gz | Fastqsanger.gz | |||
| SRR20045315_2.fastq.gz | Fastqsanger.gz | |||
| SRR20045316_1.fastq.gz | Fastqsanger.gz | |||
| SRR20045316_2.fastq.gz | Fastqsanger.gz | |||
| SRR20045317_1.fastq.gz | Fastqsanger.gz |
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2021-05-17 | MTBLS1274 | MetaboLights
Project description:Optimization of broiler chicken breast muscle protein accretion is key for the efficient production of poultry meat, whose demand is steadily increasing. In a context where antimicrobial growth promoters use is being restricted, it is important to find alternatives as well as to characterize the effect of immunological stress on broiler chicken growth. Despite of its importance, research on broiler chicken muscle protein dynamics has been mostly limited to the study of mixed protein turnover. The present study aims to characterize the effect of a bacterial challenge and the feed supplementation of a citrus and a cucumber extract on broiler chicken individual breast muscle proteins fractional synthesis rates (FSR) using a recently developed dynamic proteomics pipeline. 21 day-old broiler chickens were administered a single 2H2O dose before being culled at different timepoints. A total of 60 breast muscle protein extracts from five experimental groups (Unchallenged, Challenged, Control Diet, Diet 1 and Diet 2) were analyzed using a DDA proteomics approach. Proteomics data was filtered in order to reliably calculate multiple proteins FSR making use of a newly developed bioinformatics pipeline. Broiler breast muscle proteins FSR uniformly decreased following a bacterial challenge, this change was judged significant for 15 individual proteins, the two major functional clusters identified as well as for mixed breast muscle protein. Citrus or cucumber extract feed supplementation did not show any effect on the breast muscle protein FSR of immunologically challenged broilers. The present study has identified potential predictive markers of breast muscle growth and provided new information on broiler chicken breast muscle protein turnover which could be essential for improving the efficiency of broiler chicken meat production.
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Project description:Rational: Amino acid metabolism is crucial for inflammatory processes during atherogenesis. The endogenous amino acid homoarginine (HA) is a robust biomarker for cardiovascular outcome and mortality with high levels being protective. However, the underlying molecular mechanism remains elusive. Objective: We investigated the effect of HA supplementation on atherosclerotic plaque development with a particular focus on athero-inflammation. Methods and Results: Female apolipoprotein (Apo) E-deficient mice were supplemented with HA (14 mg/L) in drinking water starting two weeks before and continuing throughout a six week-course of Western-type diet (HA-treated). Control mice (Ctrl) received normal drinking water. HA supplementation led to a 2-fold increase in circulating HA concentrations. Plaque- and immunological phenotyping revealed that HA-treated mice exhibited a reduction in atherosclerosis in the aortic root as well as in the brachiocephalic trunk. A substantial decrease in lesion CD3+ T cells suggested a T cell-related effect of HA supplementation. Using mass spectrometry-based proteomics and subsequent pathway analysis together with conventional in vitro techniques such as flow cytometry, various migration and chemotaxis assays as well as super-resolution microscopy, we demonstrate that HA profoundly modulated the spatial organization of the T-cell actin cytoskeleton. Further mechanistic studies revealed an inhibition of T-cell activation and proliferation as well as a striking impairment of the migratory capacities of T cells in response to relevant chemokines by HA, all of which likely contribute to its atheroprotective effects. Conclusion: This study unravels a novel mechanism, by which the amino acid HA reduces atherosclerosis, namely the regulation of T-cell functions crucial for adaptive immunity. We identified that HA modulates the T-cell cytoskeleton and thereby mitigated important T-cell functions during atherogenesis. These findings provide a molecular explanation for the beneficial effects of HA in atherosclerotic cardiovascular disease.
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| EGAS00001001168 | EGA