Project description:The transcriptome changes of the ileal mucosa in suckling piglets during early postnatal life were analysed to contribute to the knowledge of a pig’s gut development. In addition, the ileal transcriptome of suckling piglets was compared with that of age-matched weaned piglets (weaned at the age of 21 days) to elucidate the effect of weaning on the developing gut. DNA microarray was used to analyse the change of transcriptome profiles and biological pathways in porcine ileum that occurred during the developmental or the weaning process.
Project description:The study investigated the impact of environment on the composition of the gut microbiota and mucosal immune development and function at gut surfaces in early and adult life. Piglets of similar genotype were reared in indoor and outdoor environments and in an experimental isolator facility. Mucosa-adherent microbial diversity in the pig ileum was characterized by sequence analysis of 16S rRNA gene libraries. Host-specific gene responses in gut ileal tissues to differences in microbial composition were investigated using Affymetrix microarray technology and Real-time PCR. Experiment Overall Design: Animals were reared on the sow at an outdoor or indoor facility. Additional piglets from the indoor facility were transferred to individual isolator units at 24 hours of age, and given a daily dose of antibiotic cocktail for the duration of the study. Piglets were weaned at day 28. From day 29 onwards, piglets were fed creep feed ad libitum. Ileal tissue samples were excised from N=6 piglets per group at day 5, 28 and 56.
Project description:Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed, food, and fuel. They can also contribute to the improved development of weaned piglets. However, their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed through carbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study was to assess the muscle metabolism of weaned piglets fed with 7% U. lactuca and 0.01% ulvan lyase supplementation, using an integrated transcriptomics (RNA-seq) and proteomics (LC-MS) approach.
Project description:The aim of this study was to characterize the metabolomic and proteomic changes in the intestinal tissue of weaned piglets fed with diets containing Spirulina as an ingredient (10% incorporation in the diet), combining such information with histomorphology data.
Project description:To investigate the effect of short distance transport on jejunal tissueof weaned piglets, We then performed gene expression profiling analysis using data obtained from RNA-seq in jejunal tissues of weaned piglets after transport and without transport
Project description:A total of 16 healthy Duroc × Landrace × Yorkshire weaned piglets (35 d of age) with an average body weight (BW) of 12.39 ± 0.24 kg were randomly allotted to two treatments with 8 piglets per treatment. The weaned piglets in the control group were fed the basal diet, and the ZEA3.0 treatment was the basal diet supplemented with 3.0 mg/kg ZEA. The basal diet prepared according to the National Research Council (NRC, 2012) The official experimental period was 32 days. After the experiment, four jejunum samples from each group were randomly selected for proteomic analysis.
Project description:Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed, food, and fuel. They can also contribute to the improved development of weaned piglets. However, their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed through carbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study was to assess the muscle metabolism of weaned piglets fed with 7% U. lactuca and 0.01% ulvan lyase supplementation, using an integrated transcriptomics and proteomics approach. Feeding piglets with seaweed and enzyme supplementation resulted in reduced macronutrient availability, leading to protein degradation through the proteasome (PSMD2), with resulting amino acids being utilized as an energy source (GOT2, IDH3B). Moreover, mineral element accumulation (iodine and bromine) contributed to increased oxidative stress, evident from elevated levels of antioxidant proteins like catalase, as a response to maintain tissue homeostasis. The upregulation of the gene AQP7, associated with the osmotic stress response, further supports these findings. Consequently, an increase in chaperone activity, including HSP90, was required to repair damaged proteins. Our results suggest that enzymatic supplementation may exacerbate the effects observed from feeding U. lactuca alone, potentially due to side effects arising from cell wall degradation during digestion.
Project description:Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed,food, and fuel. They can also contribute to the improved development of weaned piglets. However,their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed throughcarbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study wasto assess the muscle metabolism of weaned piglets fed with 7% U. lactuca and 0.01% ulvan lyasesupplementation, using an integrated transcriptomics (RNA-seq) and proteomics (LC–MS) approach.Feeding piglets with seaweed and enzyme supplementation resulted in reduced macronutrientavailability, leading to protein degradation through the proteasome (PSMD2), with resulting aminoacids being utilized as an energy source (GOT2, IDH3B). Moreover, mineral element accumulationmay have contributed to increased oxidative stress, evident from elevated levels of antioxidantproteins like catalase, as a response to maintaining tissue homeostasis. The upregulation of the geneAQP7, associated with the osmotic stress response, further supports these findings. Consequently, anincrease in chaperone activity, including HSP90, was required to repair damaged proteins. Our resultssuggest that enzymatic supplementation may exacerbate the effects observed from feeding U. lactucaalone, potentially due to side effects of cell wall degradation during digestion.
