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ABSTRACT: Transcriptome sequencing of intestinal porcine epithelial cell (IPEC-J2) between METTL3 knockdown (shMETTL3) and Control group (shCtrl)

PROVIDER: PRJNA857883 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20083846_1.fastq.gz	Fastqsanger.gz
	SRR20083846_2.fastq.gz	Fastqsanger.gz
	SRR20083847_1.fastq.gz	Fastqsanger.gz
	SRR20083847_2.fastq.gz	Fastqsanger.gz
	SRR20083848_1.fastq.gz	Fastqsanger.gz

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Comparing two intestinal porcine epithelial cell lines (IPECs): global expression patterns to characterise a in vitro model of intestinal physiology

Project description:The intestinal epithelial cell lines 1 and J2 (IPEC-1, IPEC-J2) - spontaneously immortalised cell lines from the porcine intestine - are important tools for studying intestinal function. Microarrays (GeneChip Porcine Genome Array) were used to compare the expression pattern at basal in vitro conditions. Expression analyses complemented by morphological, functional and biochemical analyses revealed that IPEC-J2 is a morphologically and functionally more differentiated cell line in comparison to IPEC-1. In addition, IPEC-2 cells are a preferential tool for in vitro studies with the focus on metabolism.

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Effect of antimicrobial peptide PR-39 on the gene expression of porcine intestinal epithelial cell line (IPEC-J2)

Project description:To gain a more complete understanding of how porcine cathelicidin PR-39 influence the porcine intestinal epithelial cells, we profiled gene expression patterns in IPEC-J2 cell line in the presence or the absence of PR-39. two groups(control and PR-39),each group has three replicates

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Transcriptome-wide m6A methylome of intestinal porcine epithelial cells (IPEC-J2) induced by deoxynivalenol

Project description:Deoxynivalenol (DON) frequently detected in a wide range of foods and feeds, inducing cytotoxicity to animals and humans. N6-methyladenosine (m6A) is an important epitranscriptomic marker with high abundance in eukaryotic mammals mRNAs. However, the role of the m6A methylomes in DON damage is still poorly understood. Here, we investigated the m6A transcriptome-wide profile in intestinal porcine epithelial cells (IPEC-J2) with and without 1000 ng/mL DON treatment via m6A sequencing and RNA sequencing. In total, 5406 new m6A peaks appeared with the disappearance of 2615 peaks in DON-induced IPEC-J2. The unique m6A-modified genes in DON-induced IPEC-J2 were associated with TNF signaling pathway. We identified 733 differentially expressed mRNA transcripts with hyper-methylated or hypo-methylated m6A peaks between DON-induced IPEC-J2 and normal IPEC-J2. Protein interaction network analysis and qPCR validation suggested that CSF2 probably acts as a promising new target for combating DON damage in IPEC-J2. Our first report of m6A transcriptome-wide map of IPEC-J2 cells presented here provides a starting roadmap for uncovering m6A functions that may affect DON infection.

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Effect of antimicrobial peptide PR-39 on the gene expression of porcine intestinal epithelial cell line (IPEC-J2)

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Project description:Transcriptome sequencing of IPEC-J2 cells between shWTAP and shCtrl group

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Global transcriptional response of porcine intestinal epithelial cell lines to Salmonella enterica serovar Typhimurium and Choleraesuis: IPI-2I infected with S. typhimurium

Project description:The GeneChip Porcine Genome Array was used to identify the transcriptional response upon either Salmonella typhimurium (ST) or Salmonella choleraesuis (SC) infection in two porcine epithelial cell lines (IPEC-J2, from jejunum and IPI-2I, from ileum) during 2 and 4 hours post infection. The objectives in this study were first, to identify the different response between the epithelial cell lines from different gut regions; second, to study how the Salmonella serotypes used could elicit a different host response; and third, to determine the effect of the time-points on the differentially gene expression.

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2013-12-20 | GSE23059 | GEO

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Transcriptome Profile of Natural Product Deoxyshikonin Induced Gene Expression in IPEC-J2

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