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A Comprehensive Survey of C. elegans Argonaute Proteins Reveals Organism-wide Gene Regulatory Networks and Functions [mutants]

ABSTRACT: A Comprehensive Survey of C. elegans Argonaute Proteins Reveals Organism-wide Gene Regulatory Networks and Functions [mutants]

PROVIDER: PRJNA860632 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20334616.fastq.gz	Fastqsanger.gz
	SRR20334617.fastq.gz	Fastqsanger.gz
	SRR20334618.fastq.gz	Fastqsanger.gz
	SRR20334619.fastq.gz	Fastqsanger.gz
	SRR20334620.fastq.gz	Fastqsanger.gz

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A Comprehensive Survey of C. elegans Argonaute Proteins Reveals Organism-wide Gene Regulatory Networks and Functions [Argonaute IP]

Project description:A Comprehensive Survey of C. elegans Argonaute Proteins Reveals Organism-wide Gene Regulatory Networks and Functions [Argonaute IP]

| PRJNA860633 | ENA

A Comprehensive Survey of C. elegans Argonaute Proteins Reveals Organism-wide Gene Regulatory Networks and Functions

Project description:A Comprehensive Survey of C. elegans Argonaute Proteins Reveals Organism-wide Gene Regulatory Networks and Functions

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A Comprehensive Survey of C. elegans Argonaute Proteins Reveals Organism-wide Gene Regulatory Networks and Functions [mutants]

Project description:Argonaute (AGO) proteins associate with small RNAs to direct their effector function on complementary transcripts. The nematode C. elegans contains an expanded family of 19 functional AGO proteins, many of which have not been fully characterized. In this work we systematically analyzed every C. elegans AGO, using CRISPR-Cas9 genome editing to introduce GFP::3xFLAG tags. We have characterized the expression patterns of each AGO throughout development, identified small RNA binding complements, and determined the effects of ago loss on small RNA populations and developmental phenotypes. Our analysis indicates stratification of subsets of AGOs into distinct regulatory modules, and integration of our data led us to uncover novel stress-induced fertility and pathogen response phenotypes due to ago loss.

2022-07-22 | GSE208701 | GEO

A Comprehensive Survey of C. elegans Argonaute Proteins Reveals Organism-wide Gene Regulatory Networks and Functions [Argonaute IP]

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A comprehensive survey of <i>C. elegans</i> argonaute proteins reveals organism-wide gene regulatory networks and functions.

Project description:Argonaute (AGO) proteins associate with small RNAs to direct their effector function on complementary transcripts. The nematode Caenorhabditis elegans contains an expanded family of 19 functional AGO proteins, many of which have not been fully characterized. In this work, we systematically analyzed every C. elegans AGO using CRISPR-Cas9 genome editing to introduce GFP::3xFLAG tags. We have characterized the expression patterns of each AGO throughout development, identified small RNA binding complements, and determined the effects of ago loss on small RNA populations and developmental phenotypes. Our analysis indicates stratification of subsets of AGOs into distinct regulatory modules, and integration of our data led us to uncover novel stress-induced fertility and pathogen response phenotypes due to ago loss.

| S-EPMC10101689 | biostudies-literature

A Comprehensive Survey of C. elegans Argonaute Proteins Reveals Organism-wide Gene Regulatory Networks and Functions

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-07-22 | GSE208702 | GEO

Comprehensive discovery of endogenous Argonaute binding sites in C. elegans

Project description:Comprehensive discovery of endogenous Argonaute binding sites in C. elegans

2009-11-24 | GSE19138 | GEO

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Project description:synthetic organism Metagenome

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Distinct Functions of Argonaute Slicer in siRNA Maturation and Heterochromatin Formation

Project description:Small-RNA (sRNA)-guided transcriptional gene silencing by Argonaute (Ago)-containing complexes is fundamental to genome integrity and epigenetic inheritance. The RNA cleavage (“Slicer”) activity of Argonaute has been implicated in both sRNA maturation and target RNA cleavage. Typically, Argonaute slices and releases the passenger strand of duplex sRNA to generate active silencing complexes, but it remains unclear whether slicing of target nascent RNAs, or other RNAi components, also contributes to downstream transcriptional silencing. Here, we develop a strategy for loading the fission yeast Ago1 with a single-stranded sRNA guide, which bypasses the requirement for slicer activity in generation of active silencing complexes. We show that slicer-defective Ago1 can mediate secondary sRNA generation, H3K9 methylation, and silencing similar to or better than wild-type and associates with chromatin more efficiently. The results define an ancient and minimal sRNA-mediated chromatin silencing mechanism, which resembles the germline-specific sRNA-dependent transcriptional silencing pathways in Drosophila and mammals. This SuperSeries is composed of the SubSeries listed below.

2016-12-19 | GSE81734 | GEO

Proteomics analysis of Escherichia coProteomics analysis of Escherichia coli BW25113 in which genes encoding Microcystis aeruginosa Argonaute (MaAgo) and Argonaute-associated Cas4 family enzyme (MaACE1) are integrated into the genome.li BW25113 in which genes encoding Microcystis aeruginosa Argonaute (MaAgo) and Argonaute-associated Cas4 family enzyme (MaACE1) are integrated into the genome.

Project description:This study was conducted in order to determine whether genome-integrated Microcystis aeruginosa Argonaute (MaAgo) and Argonaute-associated Cas4 family enzyme (MaACE1) were successfully expressed in Escherichia coli BW25113.

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