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ScRNA-seq from abdominal and gluteofemoral subcutaneous adipose tissue derived stem cells (ADSC)

ABSTRACT: scRNA-seq from abdominal and gluteofemoral subcutaneous adipose tissue derived stem cells (ADSC)

PROVIDER: PRJNA861830 | ENA |

REPOSITORIES: ENA

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scRNA-seq from abdominal and gluteofemoral subcutaneous adipose tissue derived stem cells (ADSC)

Project description:Recent studies using single cell (sc) RNAseq, identified subpopulations of adipose derived stem cells (ADSCs) within the stroma vascular fraction of human adipose tissue, potentially leading to functionally distinct adipocyte populations. We aimed to extend this analysis by exploring the heterogeneity of the ADSCs within abdominal (ABD) vs gluteofemoral (GF) subcutaneous fat depots of apple and pear shaped pre-menopausal women. Using full-length SMART-seq scRNAseq technology and a panel of known stem cell and preadipocyte markers for identification, we detected 7 subpopulations of cells in both ABD and GF-ADSCs. Two ADSC subpopulations enriched the GF depot were characterized by increased expression of genes involved in cholesterol and fatty acid metabolism. Two subpopulations enriched in the ABD depot had gene expression signatures associated with inflammation and fibrosis. The body shape comparison revealed that apple-shaped women have a unique fibrotic ADSC subpopulation in their ABD depot and a high proportion of predicted highly proliferative preadipocytes in their GF depot. We propose that these depot and body shape specific ADSC populations could contribute to the metabolic complications associated with the differential fat distribution.

2025-07-11 | GSE209618 | GEO

scRNA-Seq from stroma vascular fraction of abdominal and gluteofemoral subcutaneous white adipose tissue

Project description:scRNA-Seq from stroma vascular fraction of abdominal and gluteofemoral subcutaneous white adipose tissue

| PRJNA1077367 | ENA

Gene expression signature of subcutaneous abdominal and subcutaneous gluteal adipose tissue

Project description:To identify genes differentially expressed in abdominal and gluteal adipose tissue, we determined the mRNA transcription profile of 10 abdominal and 10 gluteal female adipose tissue sections using Agilent Whole Human Genome Microarrays. RNA of 10 subcutaneous abdominal and 10 subcutaneous gluteal fat depot samples was processed by Miltenyi Biotech GmbH (Bergisch Gladbach, Germany) and loaded on single-color Whole Human Genome 4x44K microarrays (G4112F) from Agilent Technologies.

2013-11-15 | E-GEOD-47511 | biostudies-arrayexpress

Bulk RNA sequencing of subcutaneous, abdominal and perivascular adipose derived stem cells

Project description:Previous publications demonstrated that there were anatomical, physiological, cellular, clinical and prognostic differences among adipose tissue present in subcutaneous areas, abdominal cavity and outside adventitial layer of artery. Thus, we wondered whether ASCs from subcutaneous adipose tissue, abdominal adipose tissue and perivascular adipose tissue were also different in gene expression. Here we performed bulk RNA-Seq assay for subcutaneous, abdominal and perivascular adipose derived stem cells.

2021-04-13 | GSE171946 | GEO

DNA methylation analysis of subcutaneous adipose tissue abdominal versus subcutenaous adipose tissue gluteal

Project description:In humans, adipose tissue is distributed in subcutaneous abdominal and subcutaneous gluteal depots that comprise a variety of functional differences. Whereas energy storage in gluteal adipose tissue has been shown to mediate a protective effect, an increase of abdominal adipose tissue is associated with metabolic disorders. However, the molecular basis of depot-specific characteristics is not completely understood yet. Using array-based analyses of transcription profiles, we identified a specific set of genes that was differentially expressed between subcutaneous abdominal and gluteal adipose tissue. To investigate the role of epigenetic regulation in depot-specific gene expression, we additionally analyzed genome-wide DNA methylation patterns in abdominal and gluteal depots. By combining both data sets, we identified a highly significant set of depot-specifically expressed genes that appear to be epigenetically regulated. Interestingly, the majority of these genes form part of the homeobox gene family. Moreover, genes involved in fatty acid metabolism were also differentially expressed. Therefore we suppose that changes in gene expression profiles might account for depot-specific differences in lipid composition. Indeed, triglycerides and fatty acids of abdominal adipose tissue were more saturated compared to triglycerides and fatty acids in gluteal adipose tissue. Taken together, our results uncover clear differences between abdominal and gluteal adipose tissue on the gene expression and DNA methylation level as well as in fatty acid composition. Therefore, a detailed molecular characterization of adipose tissue depots will be essential to develop new treatment strategies for metabolic syndrome associated complications. DNA methylation profiles of abdominal adipose tissue (6 samples) and gluteal adipose tissue (6 samples) were generated using Infinium methylation 450K BeadChips from Illumina (Illumina, San Diego, USA).

2013-11-15 | E-GEOD-47512 | biostudies-arrayexpress

DNA methylation analysis of subcutaneous adipose tissue abdominal versus subcutenaous adipose tissue gluteal

2013-11-15 | GSE47512 | GEO

Human Subcutaneous abdominal adipose tissue RNAseq - NYC sample

Project description:Human Subcutaneous abdominal adipose tissue RNAseq - NYC sample

| PRJNA1213843 | ENA

Genome-wide maps of chromatin state in human adipose derived stem cells isolated from abdominal (abd) and gluteofemoral (glut) subcutaneous adipose tissue

Project description:Genome-wide maps of chromatin state in human adipose derived stem cells isolated from abdominal (abd) and gluteofemoral (glut) subcutaneous adipose tissue

| PRJNA932442 | ENA

Genome-wide maps of chromatin architecture in human adipose derived stem cells isolated from abdominal (ABD) and gluteofemoral (GLU) subcutaneous adipose tissue

Project description:Genome-wide maps of chromatin architecture in human adipose derived stem cells isolated from abdominal (ABD) and gluteofemoral (GLU) subcutaneous adipose tissue

| PRJNA932714 | ENA

Genome-wide maps of chromatin state in human adipose derived stem cells isolated from abdominal (ABD) and gluteofemoral (GF) subcutaneous adipose tissue

Project description:Genome-wide maps of chromatin state in human adipose derived stem cells isolated from abdominal (ABD) and gluteofemoral (GF) subcutaneous adipose tissue

| PRJNA793019 | ENA

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