Project description:ADAR1 associated proteins of pancreatic cancer cell line BxPC3 by immunoprecipitation in response to different treatment.
Control: File DMSO In response to DMSO treatment.
Experimental: File ATRA in response to ATAR(all-trans retinoic acid)
Project description:ADAR associated proteins of pancreatic cancer cell line by immunoprecipitation in response to different treatment.
Control: File DMSO In response to DMSO treatment.(File DMSO)
Experimental: File ATRA in response to ATRA (all-trans retinoic acid)
Project description:ADAR associated proteins of pancreatic cancer cell line by immunoprecipitation in response to different treatment.
Control: File DMSO In response to DMSO treatment.(File DMSO)
Experimental: File ATRA in response to ATRA (all-trans retinoic acid)
Project description:This SuperSeries is composed of the following subset Series: GSE27334: Whole-genome gene expression level changes in P19.6 mouse embryonal carcinoma cells compared to P19.6 cells treated for 48 hours with all-trans retinoic acid GSE33067: Whole-genome mapping of MEIS1, TET1, H3K4me2 and H3K27ac in P19.6 mouse embryonal carcinoma cells and P19.6 cells treated for 48 hours with all-trans retinoic acid GSE38407: Genome-wide mapping of 5-hydroxymethylcytosine (5hmC) during differentiation of P19.6 and 3T3-L1 cells Refer to individual Series
Project description:Whole transcriptome for PRMT6 knock-out and control NT2/D1 cells with and without ATRA (all-trans retinoic acid) was sequenced. These samples were compared to each other to find differentially regulated genes and PRMT6-dependent transcriptome in pluripotency and differentiating cells.
Project description:This SuperSeries is composed of the following subset Series: GSE34672: Inhibition of the LSD1 (KDM1A) demethylase reactivates the all-trans-retinoic acid differentiation pathway in acute myeloid leukemia [Illumina HumanHT-12 gene expression array] GSE34725: Inhibition of the LSD1 (KDM1A) demethylase reactivates the all-trans-retinoic acid differentiation pathway in acute myeloid leukemia [ChIP-Seq] Refer to individual Series
Project description:Lipid droplets are fat storage organelles composed of a protein envelope which surrounds a lipid rich core. Dynamic regulation of this protein envelope underlies differential lipid droplet formation and function in diverse cell types. In melanoma, the ability to form lipid droplets has been linked to tumor progression and metastasis, but the role of specific lipid droplet proteins is unknown. To address this, we performed the first proteomic analysis of lipid droplets isolated from melanoma cells. We found that several proteins involved in retinoic acid metabolism were associated with the lipid droplet in melanoma. DHRS3, which is involved in the conversion of all-trans-retinal to all-trans-retinol, is significantly upregulated in the MITFLO/undifferentiated melanoma cell state compared to the MITFHI/melanocytic state. Increased DHRS3 expression is sufficient to drive the MITFHI/melanocytic cells to a more undifferentiated and invasive state. These changes are due to retinoic acid mediated regulation of melanocytic genes through the retinoid X receptors (RXR). Our data demonstrate that melanoma cell state can be regulated by expression of lipid droplet proteins which affect downstream retinoid signaling.